Proliferative pathways in CD1- CD3+ CD4+ CD8+ T-prolymphocytic leukemic cells: analysis with monoclonal antibodies and cytokines.

Abstract

The antigenic profile and the proliferative pathways in leukemic cells from the patient TRT with T-prolymphocytic leukemia (T-PLL) were analyzed using monoclonal antibodies (MoAbs) and cytokines. T-PLL cells expressed the phenotype CD1- CD3+ CD4+ CD8+. Incubation with the differentiating agent phorbol-12-myristate-13-acetate markedly increased the percentage of cells with the CD4- CD8+ phenotype, suggesting that leukemic cells were already committed towards a differentiated element with the CD4- CD8+ phenotype. T-PLL cells were induced to proliferate by anti-CD2 MoAb 9-1 + 9.6 and by anti-CD3 MoAb OKT3. The two pathways exhibited normal functional interactions and were susceptible to modulation by anti-HLA class I MoAbs. These results indicate that regulation of cell proliferation was preserved to a significant extent in the T-PLL cells analyzed. At variance with normal resting T cells that require previous activation to proliferate when incubated with interleukin-1 (IL-1) or interleukin-2 (IL-2), T-PLL cells proliferated vigorously when incubated with either interleukin. Furthermore, T-PLL cells proliferated when incubated with immune interferon (IFN-gamma). The latter finding parallels the enhancement by IFN-gamma of the proliferative response of lectin-activated murine T lymphocytes. These results suggest that T-PLL cells, which express a high constitutive level of c-myc mRNA, may be in an activated state. The antigenic phenotype and the characteristics of the proliferative pathways of T-PLL cells from the patient TRT are compatible with the possibility that they may be derived from an intermediate thymocyte.

• Authors: M C Turco, M De Felice, F Alfinito, A Lamberti, F Costanzo, M Giordano, V Martinelli, B Rotoli, S Ferrone, S Venuta
• Journal: Blood (Blood) Vol. 74 Issue 5 Pg. 1651-7 (Oct 1989) ISSN: 0006-4971 [Print] United States
• PMID: 2790191 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, CD
• Interleukin-1
• Interleukin-2
• Interleukin-6
• Recombinant Proteins
• Tetradecanoylphorbol Acetate

Topics

• Antibodies, Monoclonal
• Antigens, CD (analysis)
• Cell Differentiation
• Cells, Cultured
• Humans
• Interleukin-1 (pharmacology)
• Interleukin-2 (pharmacology)
• Interleukin-6 (pharmacology)
• Kinetics
• Leukemia, Prolymphocytic (immunology)
• Lymphocyte Activation (drug effects)
• Microscopy, Electron
• Recombinant Proteins (pharmacology)
• Reference Values
• T-Lymphocytes (drug effects, immunology, ultrastructure)
• Tetradecanoylphorbol Acetate (pharmacology)