Recombinant human tumour necrosis factor beta (lymphotoxin) lacks chemotactic activity for human peripheral blood neutrophils, monocytes, and T cells.

Abstract

Human recombinant tumour necrosis factor beta (rhuTNF beta)/lymphotoxin was tested for human neutrophil granulocyte (PMN), monocytes (MO), and T-cell chemotactic activity by means of a modified Boyden chamber system. Over a wide range of concentrations (10(-7)-10(-14)M)rhuTNF beta showed no chemotactic activity for PMN, MO, or T cells. In contrast, strong chemotactic migration was elicited in PMN and MO with the tripeptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) and in T cells when complement split product C5a and leukotriene B4 (LTB4) were used as chemotaxins. The results of this study indicate that rhuTNF beta/lymphotoxin is not a chemotaxin for human PMN, MO, or T lymphocytes in vitro.

• Authors: U Mrowietz, T Ternowitz, J M Schröder, E Christophers
• Journal: Scandinavian journal of immunology (Scand J Immunol) Vol. 30 Issue 3 Pg. 373-7 (Sep 1989) ISSN: 0300-9475 [Print] England
• PMID: 2789431 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemotactic Factors
• Recombinant Proteins
• Tumor Necrosis Factor-alpha
• N-Formylmethionine Leucyl-Phenylalanine

Topics

• Chemotactic Factors (pharmacology)
• Humans
• In Vitro Techniques
• Monocytes (drug effects)
• N-Formylmethionine Leucyl-Phenylalanine (pharmacology)
• Neutrophils (drug effects)
• Recombinant Proteins (pharmacology)
• T-Lymphocytes (drug effects)
• Tumor Necrosis Factor-alpha (pharmacology)