Abstract |
Monoclonal antibodies (mAb) with specificity for the T cell-associated serine proteinase-1 of the mouse (MTSP-1) were used to study expression and storage of this enzyme in T lymphocytes during lymphocytic choriomeningitis (LCM) virus infection in vivo. Immunohistochemical analysis of splenic tissue at the peak of LCM virus-specific T cell-mediated cytolytic responses, i.e., at day 7 post infection, revealed high numbers of MTSP-1+ T lymphocytes in the interfollicular T cell-dependent area of the spleen. More than 50% of Ly-2+(CD8+) cells but only low numbers of Ly-2-(CD8-) cells, previously enriched by flow cytofluorometry, contained large amounts of cytoplasmic granules which stained both with MTSP-1-specific mAb and the esterase substrate N-alpha-benzyloxycarbonyl- L-lysine thiobenzyl ester. These data demonstrate that in vivo generated LCM virus-induced cytolytic T lymphocytes develop cytoplasmic storage granules containing MTSP-1 and suggest that the mechanism of granule exocytosis is operative in vivo; possibly MTSP-1 is one effector molecule participating in the Ly-2+(CD8+) T lymphocyte-mediated control of virus infection.
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Authors | M D Kramer, U Fruth, H G Simon, M M Simon |
Journal | European journal of immunology
(Eur J Immunol)
Vol. 19
Issue 1
Pg. 151-6
(Jan 1989)
ISSN: 0014-2980 [Print] Germany |
PMID | 2784104
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, Differentiation, T-Lymphocyte
- Antigens, Ly
- Serine Endopeptidases
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Topics |
- Animals
- Antibodies, Monoclonal
(biosynthesis)
- Antigens, Differentiation, T-Lymphocyte
- Antigens, Ly
- Cytoplasmic Granules
(enzymology)
- Female
- Immunohistochemistry
- Lymphocytic Choriomeningitis
(enzymology, immunology)
- Mice
- Mice, Inbred C57BL
- Phenotype
- Rats
- Rats, Inbred Strains
- Serine Endopeptidases
(immunology, metabolism)
- Spleen
(enzymology)
- T-Lymphocytes, Cytotoxic
(enzymology)
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