Bis-pyridylethenyl benzene as novel backbone for amyloid-β binding compounds.

Abstract

Detection of cerebral β-amyloid (Aβ) by targeted contrast agents is of great interest for in vivo diagnosis of Alzheimer's disease (AD). Partly because of their planar structure several bis-styrylbenzenes have been previously reported as potential Aβ imaging agents. However, these compounds are relatively hydrophobic, which likely limits their in vivo potential. Based on their structures, we hypothesized that less hydrophobic bis-pyridylethenylbenzenes may also label amyloid. We synthesized several bis-pyridylethenylbenzenes and tested whether these compounds indeed display improved solubility and lower LogP values, and studied their fluorescent properties and Aβ binding characteristics. Bis-pyridylethenylbenzenes showed a clear affinity for Aβ plaques on both human and murine AD brain sections. Competitive binding experiments suggested a different binding site than Chrysamine G, a well-known stain for amyloid. With a LogP value between 3 and 5, most bis-pyridylethenylbenzenes were able to enter the brain and label murine amyloid in vivo with the bis(4-pyridylethenyl)benzenes showing the most favorable characteristics. In conclusion, the presented results suggest that bis-pyridylethenylbenzene may serve as a novel backbone for amyloid imaging agents.

• Authors: Rob J A Nabuurs, Varsha V Kapoerchan, Athanasios Metaxas, Sarah Hafith, Maaike de Backer, Mick M Welling, Wim Jiskoot, Adrianus M C H van den Nieuwendijk, Albert D Windhorst, Herman S Overkleeft, Mark A van Buchem, Mark Overhand, Louise van der Weerd
• Journal: Bioorganic & medicinal chemistry (Bioorg Med Chem) Vol. 24 Issue 23 Pg. 6139-6148 (12 01 2016) ISSN: 1464-3391 [Electronic] England
• PMID: 27838168 (Publication Type: Journal Article)
• Copyright: Copyright © 2016. Published by Elsevier Ltd.

Chemical References

• 1,4-bis(4'-hydroxystyryl)-2-methoxybenzene
• Amyloid beta-Peptides
• Contrast Media
• Fluorescent Dyes
• Pyridines
• Stilbenes
• Styrenes

Topics

• Amyloid beta-Peptides (chemistry)
• Animals
• Brain (diagnostic imaging, pathology)
• Contrast Media (chemical synthesis, chemistry)
• Fluorescent Dyes (chemical synthesis, chemistry)
• Humans
• Hydrophobic and Hydrophilic Interactions
• Male
• Mice, Transgenic
• Microscopy, Fluorescence
• Molecular Imaging
• Plaque, Amyloid (diagnostic imaging)
• Protein Binding
• Pyridines (chemical synthesis, chemistry)
• Solubility
• Stilbenes (chemistry)
• Styrenes (chemical synthesis, chemistry)