Abstract | AIM: METHODOLOGY: Samples were taken from 24 root canals at several clinical periods: S1 - before CMP; S2 - after CMP; S3 - after EDTA: G1 - with ultrasonic activation (n = 12) and G2 - without ultrasonic activation (n = 12). Root canals were instrumented using Mtwo rotary files. Culture techniques were used to determine the number of colony-forming units (CFU). Limulus amebocyte lysate (LAL) was used to measure endotoxin levels. Friedman's and Wilcoxon signed-rank tests were used to compare the amount of bacteria and endotoxin levels in each period (P < 0.05). RESULTS:
Endotoxins and cultivable bacteria were recovered in 100% of the initial samples (S1). CMP was effective in reducing endotoxins and bacterial load (all with P < 0.05). Higher values of endotoxin reduction were achieved with EDTA ultrasonic activation [G1, 0.02 EU mL-1 (range 0.01-0.75)] compared with the no activation group [G2, 1.13 EU mL-1 (range 0.01-8.34)] (P < 0.05). Regarding bacterial reduction, no statistically significant difference was found in S3, regardless of the group (G1, G2, P > 0.05). CONCLUSIONS:
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Authors | D R Herrera, F C Martinho, A de-Jesus-Soares, A A Zaia, C C R Ferraz, J F A Almeida, B P F A Gomes |
Journal | International endodontic journal
(Int Endod J)
Vol. 50
Issue 10
Pg. 933-940
(Oct 2017)
ISSN: 1365-2591 [Electronic] England |
PMID | 27775835
(Publication Type: Journal Article)
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Copyright | © 2016 International Endodontic Journal. Published by John Wiley & Sons Ltd. |
Chemical References |
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Topics |
- Bacteria
(drug effects, radiation effects)
- Dental Pulp Necrosis
(therapy)
- Edetic Acid
(therapeutic use)
- Endotoxins
(antagonists & inhibitors)
- Humans
- Periapical Periodontitis
(therapy)
- Root Canal Preparation
(methods)
- Stem Cells
(drug effects, radiation effects)
- Ultrasonics
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