[Cytotoxic effect of hyperthermia and nitrocaphane on malignant HEp-2 cells].

Abstract

Cytotoxic effect of hyperthermia combined with Nitrocaphane (NC) on HEp-2 cells was detected by colony formation assay. The results showed: 1. There was significant synergetic effect on survival fraction of HEp-2 cells when the heat was at 43 degrees C and 44 degrees C and NC at 0.5-4.0 micrograms/ml; 2. There was almost no effect on the cell survival when the heat was at 39 degrees C and 41 degrees C for 1 hr without NC; however, the cytotoxicity of NC (1.0 micrograms/ml) was enhanced when temperature was at 39 degrees C and 41 degrees C; 3. The sequence between heat and NC could influence the synergism. When the heat at 43 degrees C, 60 min and NC at 1.0 micrograms/ml were used simultaneously, the cytotoxicity was the strongest (SF = 0.025). When the heat was given 1-4 hr before or after the drug, the cytotoxicity was decreased. These results suggest that, over the range of 39-44 degrees C, the synergetic effects of hyperthermia and NC on malignant HEp-2 cells provide certain cell biologic basis for clinical application of local or systemic hyperthermia combined with NC.

• Authors: L G Li
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 11 Issue 1 Pg. 31-3 (Jan 1989) ISSN: 0253-3766 [Print] China
• PMID: 2776644 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents
• Nitrogen Mustard Compounds
• nitrocaphane

Topics

• Antineoplastic Agents (pharmacology)
• Hot Temperature (therapeutic use)
• Humans
• Nitrogen Mustard Compounds (pharmacology)
• Tumor Cells, Cultured (drug effects)