The expression, localization, and effect of a human interferon in plants.

Abstract

The ORF II of Cauliflower mosaic virus (CaMV) DNA was replaced with the human IFN alpha D coding sequence to yield a stable CaMV strain designated Ca524i. Inoculation of turnip (Brassica rapa cv "Just Right") with strain Ca524i DNA excised from plasmid pCa524i resulted in the production of biologically active IFN alpha D in infected plants. This was also true for its mutant (Ca562i) where one of the CYS codons was deleted. IFN alpha D produced in planta did not hamper superinfection with a single-stranded (+) sense RNA plant virus, turnip yellow mosaic virus (TYMV). Antibody gold labeling techniques and electron microscopy of infected plants showed that IFN was localized in the CaMV inclusion bodies.

• Authors: G A De Zoeten, J R Penswick, M A Horisberger, P Ahl, M Schultze, T Hohn
• Journal: Virology (Virology) Vol. 172 Issue 1 Pg. 213-22 (Sep 1989) ISSN: 0042-6822 [Print] United States
• PMID: 2773316 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Interferon Type I
• RNA, Viral
• Recombinant Proteins

Topics

• Cell Compartmentation
• Cloning, Molecular
• Genes, Viral
• Genetic Vectors
• Immunohistochemistry
• Inclusion Bodies, Viral (metabolism)
• Interferon Type I (genetics, metabolism)
• Microscopy, Electron
• Mosaic Viruses (genetics)
• Plants (genetics)
• RNA, Viral (genetics)
• Recombinant Proteins
• Viral Interference