Armadillo repeat-containing
protein 8 (ARMC8) plays an important role in regulating cell migration, proliferation, tissue maintenance, signal transduction, and
tumorigenesis. However, the expression pattern and role of ARMC8 in
osteosarcoma are still unclear. In this study, our aims were to examine the effects of ARMC8 on
osteosarcoma and to explore its underlying mechanism. Our results demonstrated that ARMC8 was overexpressed in
osteosarcoma cell lines. Knockdown of ARMC8 significantly inhibited
osteosarcoma cell proliferation in vitro and markedly inhibited xenograft
tumor growth in vivo. ARMC8 silencing also suppressed the epithelial-mesenchymal transition (EMT) phenotype, as well as inhibited the migration and invasion of
osteosarcoma cells. Furthermore, knockdown of ARMC8 obviously inhibited the expression of β-
catenin, c-Myc, and
cyclin D1 in MG-63 cells. In conclusion, this report demonstrates that ARMC8 silencing inhibits proliferation and invasion of
osteosarcoma cells. Therefore, ARMC8 may play an important role in the development and progression of human
osteosarcoma and may represent a novel therapeutic target in the treatment of
osteosarcoma.