Abstract |
Dietary isothiocyanates abundant as glucosinolate precursors in many edible cruciferous vegetables are effective for prevention of cancer in chemically-induced and transgenic rodent models. Some of these agents, including phenethyl isothiocyanate ( PEITC), have already advanced to clinical investigations. The primary route of isothiocyanate metabolism is its conjugation with glutathione (GSH), a reaction catalyzed by glutathione S-transferase (GST). The pi class GST of subunit type 1 (hGSTP1) is much more effective than the alpha class GST of subunit type 1 (hGSTA1) in catalyzing the conjugation. Here, we report the crystal structures of hGSTP1 and hGSTA1 each in complex with the GSH adduct of PEITC. We find that PEITC also covalently modifies the cysteine side chains of GST, which irreversibly inhibits enzymatic activity.
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Authors | Vandana Kumari, Marzena A Dyba, Ryan J Holland, Yu-He Liang, Shivendra V Singh, Xinhua Ji |
Journal | PloS one
(PLoS One)
2016
Vol. 11
Issue 9
Pg. e0163821
ISSN: 1932-6203 [Electronic] United States |
PMID | 27684484
(Publication Type: Journal Article)
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