Isolation of galactosaminoglycan moiety (CO-N) from protein-bound polysaccharide of Cordyceps ophioglossoides and its effects against murine tumors.

Abstract

A galactosaminoglycan moiety was obtained from an antitumor polysaccharide fraction (SN-C) isolated from Cordyceps ophioglossoides culture. SN-C was subjected to sonication, then a protein-bound galactosaminoglycan (CO-N) was isolated specifically by precipitation with 10% ammonium hydroxide. When given intraperitoneally to mice, CO-N inhibited the proliferation of sarcoma 180 cells inoculated into the peritoneal cavity and exhibited a marked life-prolonging effect against ascitic tumors such as Ehrlich carcinoma and IMC carcinoma. CO-N also showed an inhibitory effect against solid Ehrlich carcinoma when given intratumorally and significantly inhibited the growth of a syngeneic solid tumor (MM46 mammary carcinoma) upon intravenous administration at a low dose. CO-N showed a cytocidal effect against cultured cells of IMC and P388D1 in vitro. Flow cytometric analysis demonstrated that fluorescein isothiocyanate-CO-N binds to the surface of Ehrlich cells.

• Authors: T Ohmori, K Tamura, K Fukui, G Kawanishi, M Mitsuyama, K Nomoto, T Miyazaki
• Journal: Chemical & pharmaceutical bulletin (Chem Pharm Bull (Tokyo)) Vol. 37 Issue 4 Pg. 1019-22 (Apr 1989) ISSN: 0009-2363 [Print] Japan
• PMID: 2766404 (Publication Type: Journal Article)

Chemical References

• Antibiotics, Antineoplastic
• Polysaccharides
• galactosaminoglycan

Topics

• Animals
• Antibiotics, Antineoplastic (isolation & purification, pharmacology)
• Hypocreales (metabolism)
• Male
• Mice
• Mice, Inbred C3H
• Mice, Inbred ICR
• Neoplasms, Experimental (drug therapy)
• Polysaccharides (analysis, isolation & purification, pharmacology)