A model of acute regional myocardial ischemia and reperfusion in the rat.

Abstract

Studies were conducted in 76 rats to describe and validate a new closed-chest in vivo model for acute ischemia and reperfusion of the left coronary artery. Radiolabeled microsphere distribution in six rat hearts confirmed a significant reduction in arteriolar flow at the center of the ischemic zone (93% reduction of total myocardial counts compared to nonischemic region, P less than 0.01) after 7 min of occlusion. Arteriolar flow returned to control values upon reperfusion. While hemodynamic parameters in 10 rats during 7 min of occlusion and 7 min of reperfusion were monitored, end diastolic pressures increased significantly (P less than 0.01) during occlusion. Finally, the utility of this rat model was demonstrated in a study of contrast enhanced magnetic resonance imaging (N = 4). Normal myocardium could not be differentiated from acutely ischemic myocardium on noncontrast-enhanced MR images. After 5 min of myocardial ischemia and following contrast administration (albumin-Gd-DTPA), the ischemic zone appeared less enhanced than normal myocardium. Upon release of the occluder the left ventricular free wall once again yielded a homogeneous signal similar to that of the normal myocardium.

• Authors: R E Sievers, U Schmiedl, C L Wolfe, M E Moseley, W W Parmley, R C Brasch, M J Lipton
• Journal: Magnetic resonance in medicine (Magn Reson Med) Vol. 10 Issue 2 Pg. 172-81 (May 1989) ISSN: 0740-3194 [Print] United States
• PMID: 2761378 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Topics

• Animals
• Coronary Disease (pathology, physiopathology)
• Disease Models, Animal
• Magnetic Resonance Imaging
• Microspheres
• Myocardial Reperfusion
• Myocardium (pathology)
• Rats
• Rats, Inbred Strains