The role of IL-1β and glutamate in the effects of lipopolysaccharide on the hippocampal electrical kindling of seizures.

Abstract

In our study, we used rapid electrical hippocampal kindling and in vivo microdialysis methods to assess the involvement of inflammatory mediators: lipopolysaccharide (LPS) and proinflammatory interleukin-1β (IL-1β) in mechanisms of epileptogenesis. We observed, that both, LPS and IL-1β, administered into stimulated hippocampus, accelerated kindling process. LPS also increased the expression of IL-1β in stimulated hippocampus in kindled rats. In vivo acute LPS perfusion, via a microdialysis cannula implanted into the naïve rat's hippocampus, produced an increase in extracellular glutamate release. We suppose, that particularly IL-1β action and increased glutamate concentration may significantly contribute to LPS effects on kindling development.

• Authors: Karolina Kołosowska, Piotr Maciejak, Janusz Szyndler, Danuta Turzyńska, Alicja Sobolewska, Adam Płaźnik
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 298 Pg. 146-52 (09 15 2016) ISSN: 1872-8421 [Electronic] Netherlands
• PMID: 27609288 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Cytokines
• Interleukin-1beta
• Lipopolysaccharides
• Glutamic Acid
• gamma-Aminobutyric Acid

Topics

• Analysis of Variance
• Animals
• Cytokines (metabolism)
• Disease Models, Animal
• Electric Stimulation
• Electroencephalography
• Enzyme-Linked Immunosorbent Assay
• Glutamic Acid (metabolism)
• Hippocampus (drug effects, metabolism)
• Interleukin-1beta (metabolism, pharmacology)
• Kindling, Neurologic (drug effects)
• Lipopolysaccharides (toxicity)
• Male
• Microdialysis
• Rats
• Rats, Wistar
• Seizures (chemically induced)
• Time Factors
• gamma-Aminobutyric Acid (metabolism)