Huaier restrains proliferative and invasive potential of human hepatoma SKHEP-1 cells partially through decreased Lamin B1 and elevated NOV.

Abstract

Hepatocellular carcinoma (HCC) is one of the most common cause of malignancy-related mortality worldwide. It is urgently needed to develop potential drugs with good efficacy and low toxicity for HCC treatment. The anti-tumor effect of Traditional Chinese Medicine (TCM) has received increasing attention worldwide. Trametes robiniophila Murr. (Huaier) has been used in TCM for approximately 1,600 years. Clinically, Huaier has satisfactory therapeutic effects in cancer treatment, especially in HCC. However, the mechanisms underlying the anti-cancer effect of Huaier remain ill defined. Herein we have demonstrated that Huaier dramatically inhibited cell proliferation and induced apoptosis in human hepatoma cell line SKHEP-1. Importantly, Huaier restrained the metastatic capability of SKHEP-1 cells. Mechanistically, down-regulation of Lamin B1 and up-regulation of Nephroblastoma overexpressed (NOV) were at least partially responsible for the inhibitory effect of Huaier on the proliferative and invasive capacity of SKHEP-1 cells. Our finding provided new insights into mechanisms of anti-HCC effect of Huaier and suggested a new scientific basis for clinical medication.

• Authors: Zhongdong Hu, Ailin Yang, Guozhu Su, Yunfang Zhao, Ying Wang, Xingyun Chai, Pengfei Tu
• Journal: Scientific reports (Sci Rep) Vol. 6 Pg. 31298 (08 09 2016) ISSN: 2045-2322 [Electronic] England
• PMID: 27503760 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCN3 protein, human
• Complex Mixtures
• Lamin Type B
• Nephroblastoma Overexpressed Protein
• huaier

Topics

• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (drug therapy, metabolism)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation
• Cell Survival (drug effects)
• Complex Mixtures (pharmacology)
• Gene Expression Regulation, Neoplastic
• Humans
• Lamin Type B (metabolism)
• Liver Neoplasms (drug therapy, metabolism)
• Medicine, Chinese Traditional
• Neoplasm Invasiveness
• Nephroblastoma Overexpressed Protein (metabolism)
• Oligonucleotide Array Sequence Analysis
• Trametes (chemistry)