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DNA polymerase activities in differentiating mouse neuroblastoma N-18 cells.

Abstract
The activities of two DNA polymerases (DNA nucleotidyltransferases) were characterized in mouse neuroblastoma clone N-18 on the basis of their apparent molecular weights (determined by sucrose density gradient centrifugation: polymerase-alpha, 7.5-8 S; polymerase-beta, 3-4 S) and relative inhibition by sulfhydryl-blocking agents. N-Ethylmaleimide (10 mM) and iodoacetamide (1.5 mM) inhibited DNA polymerase-alpha activity completely, whereas only 35-40% inhibition was observed for DNA polymerase-beta under similar conditions. DNA polymerase-alpha activity was reduced 50-70% in N-18 cells that had been induced to differentiate by 4 micro M bromodeoxyuridine, and the low molecular weight DNA polymerase-beta activity remain unchanged. With activated calf thymus DNA as template, only DNA polymerase-alpha activity was stimulated in the presence of added ribonucleotides and purified Escherichia coli RNA polymerase.
AuthorsP Bhattacharya, S Basu
JournalProceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 75 Issue 3 Pg. 1289-93 (Mar 1978) ISSN: 0027-8424 [Print] United States
PMID274718 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Neoplasm
  • Sulfhydryl Reagents
  • DNA-Directed RNA Polymerases
  • DNA Polymerase I
  • DNA Polymerase II
  • DNA-Directed DNA Polymerase
  • Bromodeoxyuridine
Topics
  • Bromodeoxyuridine (pharmacology)
  • Cell Differentiation (drug effects)
  • Cell Line
  • DNA Polymerase I (antagonists & inhibitors, metabolism)
  • DNA Polymerase II (antagonists & inhibitors, metabolism)
  • DNA, Neoplasm (biosynthesis)
  • DNA-Directed DNA Polymerase (metabolism)
  • DNA-Directed RNA Polymerases (metabolism)
  • Neoplasms, Experimental
  • Neuroblastoma (enzymology)
  • Sulfhydryl Reagents (pharmacology)

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