Formation of 1-beta-D-arabinofuranosylcytosine diphosphate choline in neoplastic and normal cells.

Abstract

1-beta-D-Arabinofuranosylcytosine diphosphate choline was formed from 1-beta-D-arabinofuranosylcytosine (ara-C) during incubation in vitro of peripheral myeloblasts from patients with acute myelogenous leukemia and cultured cells (nonleukemic human lymphocytes, mouse lymphoma L5178Y, and HeLa); as well, 1-beta-D-arabinofuranosylcytosine diphosphate choline was formed in vivo in mouse leukemia L1210 cells and mouse liver. 3-Deazauridine enhanced the anabolism of ara-C in nonleukemic lymphocytes in vitro and leukemia L1210 cells in vivo but did not influence ara-C anabolism in the other cell types. In acute myelogenous leukemia myeloblasts incubated in vitro with ara-C, concentrations of 1-beta-D-arabinofuranosylcytosine 5'-triphosphate were maximal after 8 hr of incubation and formation of the latter preceded that of 1-beta-D-arabinofuranosylcytosine diphosphate choline.

• Authors: G J Lauzon, A R Paterson, A W Belch
• Journal: Cancer research (Cancer Res) Vol. 38 Issue 6 Pg. 1730-3 (Jun 1978) ISSN: 0008-5472 [Print] United States
• PMID: 274175 (Publication Type: Journal Article)

Chemical References

• Cytarabine
• 3-Deazauridine
• Cytidine Diphosphate Choline
• Choline

Topics

• 3-Deazauridine (pharmacology)
• Animals
• Choline (analogs & derivatives)
• Cytarabine (analogs & derivatives, biosynthesis, metabolism, pharmacology)
• Cytidine Diphosphate Choline (analogs & derivatives, biosynthesis)
• Drug Synergism
• Female
• HeLa Cells (metabolism)
• Leukemia L1210 (metabolism)
• Leukemia, Experimental (drug therapy, metabolism)
• Leukemia, Myeloid, Acute (metabolism)
• Liver (metabolism)
• Lymphocytes (metabolism)
• Mice
• Mice, Inbred Strains