[Expression of two transcript variants of long noncoding RNA C6orf176 in non-small cell lung cancer and its clinical significance].

Abstract

OBJECTIVE:

To explore two transcript variants expression of long noncoding RNA C6orf176 in non-small cell lung cancer (NSCLC) and the clinical pathological significance. 


METHODS:

The expressions of transcript variant 1 (TV1) and transcript variant 2 (TV2) of long noncoding RNA C6orf176 in 57 NSCLC and adjacent cancerous tissues were examined by qPCR with β-actin as internal control.


RESULTS:

Based on the results of qPCR, for C6orf176-TV1, 42 cases were down-regulated and 15 cases were up-regulated. The C6orf176-TV1 level was correlated to the grade of differentiation (P<0.05) but it was not correlated with gender, age, smoking history, tumor type and TNM stage. The expression of C6orf176-TV1 had a potential value in diagnosis of NSCLC by receiver operator characteristic (ROC) curve. Area under curve (AUC) of ROC curve was 0.708 (95% CI 0.615 to 0. 802). The sensitivity and specificity were 51% and 88%, respectively. For C6orf176-TV2, 39 cases were down-regulated and 18 cases were up-regulated. The C6orf176-TV2 level was correlated with the grade of differentiation (P<0.05) but it was not correlated with gender, age, tumor size, smoking history and TNM stage. C6orf176-TV2 level had value in diagnosis of NSCLC by ROC curve. AUC of ROC curve was 0.64 (95% CI 0.531 to 0.749). The sensitivity and specificity were 49% and 75%, respectively. Of the 57 specimens, 53 cases were simultaneous up or down-regulation of C6orf176-TV1 and C6orf176-TV2. The correlation coefficient was 0.99.


CONCLUSION:

The expression of C6orf176-TV1 or C6orf176-TV2 is down-regulated in NSCLC and it is correlated with the grade of differentiation. It may act as a diagnosis indicator for NSCLC patients.

• Authors: Jun Wang, Zhenzi Peng, Yeping Dong, Dan He, Chaojun Duan, Xinying Li
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 41 Issue 6 Pg. 560-5 (Jun 28 2016) ISSN: 1672-7347 [Print] China
• PMID: 27374438 (Publication Type: Journal Article)

Chemical References

• Actins
• RNA, Long Noncoding

Topics

• Actins
• Area Under Curve
• Carcinoma, Non-Small-Cell Lung
• Cell Differentiation
• Down-Regulation
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms
• RNA, Long Noncoding
• ROC Curve
• Real-Time Polymerase Chain Reaction
• Smoking
• Transcription, Genetic
• Up-Regulation