The Safety Property of β-D-Mannuronic Acid (M2000) as a Novel Immunosuppressive Agent on Differentiation, Maturation and Function of Human Dendritic Cells.

Abstract

The study's background and aim: In this investigation, the safety property of M2000 (β-D-mannuronic acid) on differentiation, maturation and function of dendritic cells, was determined. β-D-mannuronic acid, as a novel immunosuppressive and anti-inflammatory agent, has been tested in various experimental models. In addition, DC-based immunosuppressive drugs can suppress the progression of autoimmune diseases, although, their notable side effects in increasing the risk of infectious diseases and cancers should be considered.

MATERIALS AND METHODS:

The effect of M2000 on differentiation, maturation and function of dendritic cells was examined. To investigate how M2000 affects human dendritic cells (DC) in a defined inflammatory environment, human peripheral blood mononuclear cells (PBMC) were isolated from healthy blood and monocytes were purified using anti-CD14 microbeads. Monocytes were incubated with M2000 in two different doses (6 and 12 J.g/well) along with adding the granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 for inducing monocytes to immature DC and lipopolysaccharide for running DC maturation. The differentiation, maturation and function of dendritic cells were examined with flow cytometry and ELISA method.

RESULT:

The results demonstrate that M2000 has no significant side on differentiation, maturation and function of dendritic cells in immature DC and mature DC process in vitro.

CONCLUSION:

Our findings show that β-D-mannuronic acid (m2000) as a safe agent had no adverse effect on differentiation, maturation and function of dendritic cells which might be recommended as a novel immunosuppressive agent with no or fewer side effects in increasing the risk of infectious diseases and cancers.

• Authors: Nazanin A Fard, Nakisa Tabrizian, Reza Mirzaei, Nasrin Motamed, Farzaneh T Zavareh, Abbas Mirshafiey
• Journal: Current drug discovery technologies (Curr Drug Discov Technol) Vol. 13 Issue 3 Pg. 164-169 ( 2016) ISSN: 1875-6220 [Electronic] United Arab Emirates
• PMID: 27335103 (Publication Type: Journal Article)

Chemical References

• Cytokines
• Hexuronic Acids
• Immunosuppressive Agents
• mannuronic acid

Topics

• Cell Differentiation (drug effects)
• Cells, Cultured
• Cytokines (metabolism)
• Dendritic Cells (drug effects, metabolism, physiology)
• Hexuronic Acids (pharmacology)
• Humans
• Immunosuppressive Agents (pharmacology)