Alterations in cathepsin L expression in lung cancers.
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| Abstract |
We herein investigated the potential role of cathepsin L in lung carcinogenesis. Lung cancer cell lines and surgically resected tumors were examined for the expression of the cathepsin L protein and copy number alterations in its gene locus. Cathepsin L was stably expressed in bronchiolar epithelial cells. Neoplastic cells expressed cathepsin L at various levels, whereas its expression was completely lost in most of the lung cancer cell lines (63.6%, 7/11) examined. Furthermore, expression levels were lower in a large fraction of lung tumors (69.5%, 139/200) than in bronchiolar epithelia. The expression of cathepsin L was lost in some tumors (16.0%, 32/200). In adenocarcinomas, expression levels were significantly lower in high-grade tumors than in low-grade tumors (one-way ANOVA, P < 0.0500). Copy number alterations were found in 18.0% (36 [32 gain + 4 loss] /200) of lung tumors. No relationship existed between cathepsin L protein expression levels and the copy number of its gene locus (Spearman's rank-order correlation, P = 0.3096). Collectively, these results suggest that the down-regulated expression of cathepsin L, which is caused by an undefined mechanism other than copy number alterations, is involved in the progression of lung adenocarcinomas.
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| Authors | Koji Okudela, Hideaki Mitsui, Tetsukan Woo, Hiromasa Arai, Takehisa Suzuki, Mai Matsumura, Yoko Kojima, Shigeaki Umeda, Yoko Tateishi, Munetaka Masuda, Kenichi Ohashi |
| Journal | Pathology international (Pathol Int) Vol. 66 Issue 7 Pg. 386-92 (Jul 2016) ISSN: 1440-1827 [Electronic] Australia |
| PMID | 27327955 (Publication Type: Journal Article) |
| Copyright | © 2016 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd. |
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