Abstract | BACKGROUND: RESULTS: We performed a reaction between FITC and PAMAM G4.5 dendrimers which were previously derivatized with piperazine (linker molecule), characterized them by (1)H NMR ( proton nuclear magnetic resonance) spectroscopy and MALDI-TOF (matrix-assisted laser desorption/ionization- time-of-flight) mass spectrometry. The experimental data indicated that 2 FITC molecules could be bound covalently at the PAMAM G4.5 dendrimer surface, with 17 FITC molecules probably occluded in PAMAM dendrimers cavity. PAMAM- FITC dendrimer (PAMAM G4.5-piperazinyl-FITC dendrimer) size distribution was evaluated by DLS (dynamic light scattering) and TEM (transmission electron microscopy). The nanoparticle hydrodynamic size was 96.3 ± 1.4 nm with a PdI (polydispersion index) of 0.0296 ± 0.0171, and the size distribution measured by TEM was 44.2 ± 9.2 nm. PAMAM- FITC dendrimers were neither cytotoxic in 4T1 cells nor hemolytic up to 24 h of incubation. In addition, they were uptaken in vitro by 4T1 cells and in vivo by BALB/c mice breast tumors. PAMAM G4.5-piperazinyl-FITC dendrimer intracellular distribution was observed through histologic analysis of the tumor by laser confocal microscopy. CONCLUSION:
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Authors | Natalia Oddone, Nicole Lecot, Marcelo Fernández, Alejandra Rodriguez-Haralambides, Pablo Cabral, Hugo Cerecetto, Juan Claudio Benech |
Journal | Journal of nanobiotechnology
(J Nanobiotechnology)
Vol. 14
Issue 1
Pg. 45
(Jun 13 2016)
ISSN: 1477-3155 [Electronic] England |
PMID | 27297021
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Dendrimers
- Drug Carriers
- Fluorescent Dyes
- Nylons
- PAMAM-G4
- Fluorescein-5-isothiocyanate
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Topics |
- Animals
- Antineoplastic Agents
(administration & dosage, pharmacokinetics)
- Breast
(metabolism)
- Breast Neoplasms
(metabolism)
- Cell Line, Tumor
- Dendrimers
(metabolism)
- Drug Carriers
(metabolism)
- Female
- Fluorescein-5-isothiocyanate
(administration & dosage, pharmacokinetics)
- Fluorescent Dyes
(administration & dosage, pharmacokinetics)
- Mice
- Mice, Inbred BALB C
- Nylons
(metabolism)
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