[Absorption and transition of [8-14C]adenosine and 2',3'-O-isopropylidene adenosine by Zaidel hepatomas and their homogenates].

Abstract

Transformation and uptake of [8-14C]-adenosine and its synthetic analog 2',3'-O-isopropylideneadenosine was studied in Zajdel hepatoma cells and their homogenates. Uptake and deamination of adenosine and 2',3'-O-isopropylideneadenosine by Zajdel hepatoma cells proceed differently. A small part of adenosine is phosphorylated and then it is included into biosynthesis of polymer substances. The uptake and deamination of 2',3'-O-isopropylideneadenosine by hepatoma cells occurs more intensively than uptake and deamination of adenosine. The formed 2',3'-O-isopropylideneadenosine is not splitted by purine nucleoside phosphorylase and is accumulated in cells in the incubation medium that lead to cell death. The same rate of 2',3'-O-isopropylideneadenosine deamination in cells and their homogenates indicates its high penetrability through plasma membranes. The high uptake of 2',3'-O-isopropylideneadenosine contrary to adenosine leads to deaggregation of cells and their destruction.

• Authors: I D Golovatskiĭ, L I Petlichnaia, A F Lashkaĭ
• Journal: Ukrainskii biokhimicheskii zhurnal (1978) (Ukr Biokhim Zh (1978)) 1989 Mar-Apr Vol. 61 Issue 2 Pg. 64-9 ISSN: 0201-8470 [Print] Ukraine
• Vernacular Title: Pogloshchenie i prevrashchenie [8-14C]adenozina i 2',3'-O-izopropilidenadenozina kletkami i gomogenatami kletok gepatomy Zaĭdelia.
• PMID: 2728115 (Publication Type: English Abstract, Journal Article)

Chemical References

• Purines
• 2',3'-isopropylideneadenosine
• Adenosine

Topics

• Adenosine (analogs & derivatives, metabolism)
• Animals
• Cells, Cultured
• Liver Neoplasms, Experimental (metabolism)
• Purines (metabolism)
• Rats
• Tumor Cells, Cultured (metabolism)