Chemotherapeutic effect of Berberis integerrima hydroalcoholic extract on colon cancer development in the 1,2-dimethyl hydrazine rat model.

Abstract

The aim of this study was to investigate the efficacy of a Berberis integerrima hydroalcoholic extract as a chemotherapeutic agent in colon carcinogenesis in the rat induced by 1,2-dimethyl hydrazine (DMH). Male Wistar rats were divided into five groups: a negative control group without DMH treatment; a control group injected DMH (20 mg/kg b.w); two groups receiving B. integerrima extract (50 and 100 mg/kg b.w), concomitant with injected DMH, as chemotherapeutic groups; a positive control group receiving 5-fluorouracil (5-FU) along with DMH. The effects of the extracts were determined by assessment of hepatic malondialdehyde (MDA), glutathione (GSH), ferric reducing ability of plasma (FRAP), and the activities of hepatic glutathione S-transferase and cytochrome P450 (GST and CYP450). Additionally, colon tissues were assessed for colonic β-catenin and histopathological analysis. In DMH-treated rats, the extracts partially normalized the levels of FRAP, CYP450, β-catenin, and GST. Likewise, formation of aberrant crypt foci (ACF) in colon tissue of DMH-treated was reduced by the extracts. Thus, the extracts possess chemotherapeutic activity against colon carcinogenesis.

• Authors: Mohammad R Mohammadi Malayeri, Abolfazl Dadkhah, Faezeh Fatemi, Salome Dini, Fatemeh Torabi, Mohammad M Tavajjoh, Javad Rabiei
• Journal: Zeitschrift fur Naturforschung. C, Journal of biosciences (Z Naturforsch C J Biosci) 2016 Vol. 71 Issue 7-8 Pg. 225-32 ISSN: 0939-5075 [Print] Germany
• PMID: 27232632 (Publication Type: Journal Article)

Chemical References

• Carcinogens
• Plant Extracts
• beta Catenin
• Water
• Ethanol
• Malondialdehyde
• Cytochrome P-450 Enzyme System
• Glutathione Transferase
• Glutathione
• 1,2-Dimethylhydrazine

Topics

• 1,2-Dimethylhydrazine
• Aberrant Crypt Foci (drug therapy, metabolism)
• Animals
• Berberis (chemistry)
• Carcinogens
• Colon (drug effects, metabolism, pathology)
• Colonic Neoplasms (chemically induced, drug therapy, metabolism)
• Cytochrome P-450 Enzyme System (metabolism)
• Disease Models, Animal
• Ethanol (chemistry)
• Glutathione (metabolism)
• Glutathione Transferase (metabolism)
• Liver (drug effects, metabolism, pathology)
• Male
• Malondialdehyde (metabolism)
• Phytotherapy
• Plant Extracts (chemistry, pharmacology)
• Rats, Wistar
• Treatment Outcome
• Water (chemistry)
• beta Catenin (metabolism)