Search for nicotinic acetylcholine receptors on human leukocytes: absence of alpha-bungarotoxin binding in studies of healthy individuals and myasthenia gravis patients.

Abstract

To investigate whether nicotinic acetylcholine receptors might be present on blood mononuclear cells we studied the binding [125I]alpha-bungarotoxin to mononuclear cells from three normal controls and seven myasthenia gravis patients. The medulloblastoma cell line, TE671, which expresses a functional nicotinic acetylcholine receptor having pharmacological properties similar to that of skeletal muscle receptor, was used as a positive control for alpha-bungarotoxin binding. None of the mononuclear cell samples studied exhibited specific binding of alpha-bungarotoxin.

• Authors: R L Kaufman, J Oger
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 23 Issue 1 Pg. 83-7 (Jun 1989) ISSN: 0165-5728 [Print] Netherlands
• PMID: 2723042 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bungarotoxins
• Chrna7 protein, human
• Receptors, Cholinergic
• Receptors, Nicotinic
• alpha7 Nicotinic Acetylcholine Receptor

Topics

• Bungarotoxins (metabolism)
• Cell Line
• Humans
• Leukocytes, Mononuclear (metabolism)
• Medulloblastoma (metabolism)
• Myasthenia Gravis (metabolism)
• Receptors, Cholinergic (analysis)
• Receptors, Nicotinic (analysis)
• alpha7 Nicotinic Acetylcholine Receptor