Determination of Antibody-Drug Conjugate Released Payload Species Using Directed in Vitro Assays and Mass Spectrometric Interrogation.

Abstract

Antibody-drug conjugates (ADC) are currently an active area of research, focused primarily on oncology therapeutics, but also to a limited extent on other areas such as infectious disease. The success of this type of targeted drug delivery is dependent upon many factors, one of which is the performance of the linker in releasing an active drug moiety under the appropriate conditions. As a tool in the development of linker/payload chemistry, we have developed an in vitro method for the identification of payload species released from ADCs in the presence of lysosomal enzymes. This method utilizes commercially available human liver S9 fraction as the source of these enzymes, and this has certain advantages over lysosomal fractions or purified enzymes. This article describes the characterization and performance of this assay with multiple ADCs composed of known and novel linkers and payloads. Additionally, we report the observation of incomplete degradation of mAb protein chains by lysosomal enzymes in vitro, believed to be the first report of this phenomenon involving an ADC therapeutic.

• Authors: Andrew J Bessire, T Eric Ballard, Manoj Charati, Justin Cohen, Michael Green, My-Hanh Lam, Frank Loganzo, Birte Nolting, Betsy Pierce, Sujiet Puthenveetil, Lee Roberts, Klaas Schildknegt, Chakrapani Subramanyam
• Journal: Bioconjugate chemistry (Bioconjug Chem) Vol. 27 Issue 7 Pg. 1645-54 (Jul 20 2016) ISSN: 1520-4812 [Electronic] United States
• PMID: 27206324 (Publication Type: Journal Article)

Chemical References

• Drug Carriers
• Immunoconjugates
• Cathepsin B

Topics

• Animals
• Cathepsin B (metabolism)
• Cell Line, Tumor
• Drug Carriers (chemistry, metabolism)
• Drug Liberation
• Humans
• Immunoconjugates (chemistry)
• Liver (cytology)
• Lysosomes (enzymology)
• Mass Spectrometry
• Mice
• Rats