Effective elimination of liver cancer stem-like cells by CD90 antibody targeted thermosensitive magnetoliposomes.

Abstract

AIM:

To investigate the use of thermosensitive magnetoliposomes (TMs) loaded with magnetic iron oxide (Fe3O4) and the anti-cancer stem cell marker CD90 (CD90@TMs) to target and kill CD90+ liver cancer stem cells (LCSCs).

METHODS:

The hepatocellular carcinoma cell line Huh7 was used to separate CD90+ LCSCs by magnetic-activated cell sorting. CD90@TMs was characterized and their ability to target CD90+ LCSCs was determined. Experiments were used to investigate whether CD90@TMs combined with magnetic hyperthermia could effectively eliminate CD90+ LCSCs.

RESULTS:

The present study demonstrated that CD90+ LCSCs with stem cells properties were successfully isolated. We also successfully prepared CD90@TMs that was almost spherical and uniform with an average diameter of 130±4.6 nm and determined that magnetic iron oxide could be incorporated and retained a superparamagnetic response. CD90@TMs showed good targeting and increased inhibition of CD90+ LCSCs in vitro and in vivo compared to TMs.

CONCLUSIONS:

CD90@TMs can be used for controlled and targeted delivery of anticancer drugs, which may offer a promising alternative for HCC therapy.

• Authors: Rui Yang, Li Y An, Qin F Miao, Feng M Li, Yong Han, Hui X Wang, Dang P Liu, Rong Chen, Sha Q Tang
• Journal: Oncotarget (Oncotarget) Vol. 7 Issue 24 Pg. 35894-35916 (Jun 14 2016) ISSN: 1949-2553 [Electronic] United States
• PMID: 27145285 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Thy-1 Antigens

Topics

• Animals
• Antibodies (immunology, pharmacology)
• Apoptosis (drug effects, immunology)
• Carcinoma, Hepatocellular (drug therapy, immunology, metabolism)
• Cell Line
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Humans
• Immunomagnetic Separation (methods)
• Liver Neoplasms (drug therapy, immunology, metabolism)
• Mice, Inbred NOD
• Mice, SCID
• Neoplastic Stem Cells (drug effects, immunology, metabolism)
• Temperature
• Thy-1 Antigens (immunology, metabolism)
• Xenograft Model Antitumor Assays (methods)