[Downregulation of proteinase activated receptor 4 inhibits migration of SW620 human colorectal cancer cells].

Abstract

OBJECTIVE:

To establish the human colorectal cancer cell model SW620/PAR4D with inducible suppression of proteinase activated receptor 4 (PAR4) expression, and investigate the role PAR4 plays in the proliferation and migration of cancer cells.

METHODS:

A human colorectal cancer cell line with tetracycline-inducible expression regulatory system, namely SW620/Tet-on, was established; inducible expression lentiviral vector with artificial microRNA targeting PAR4, pLVX-Tight-Puro-PAR4-miR, was constructed and transfected into SW620/Tet-on to make an inducible PAR4-suppressed cell model SW620/PAR4D. Western blotting was used to confirm the suppression of PAR4 expression after the doxycycline (DOX) treatment. CCK-8 assay was used to evaluate the impact of suppressed PAR4 expression on cell proliferation, and wound-healing assay was used to analyze the migration of the cells.

RESULTS:

The SW620/PAR4D cell model was established successfully. Suppression of PAR4 expression by DOX treatment had no significant impact on the growth/proliferation of SW620/PAR4D cells, but markedly inhibited the cell migration.

CONCLUSION:

Suppression of PAR4 expression has no significant effect on the proliferation of SW620 cells, but can inhibit the migration of the cells.

• Authors: Lihong Chen, Chunchun Li, Yuqiong Xie, Jingjia Ye, Jiang Cao
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 32 Issue 5 Pg. 609-14 (May 2016) ISSN: 1007-8738 [Print] China
• PMID: 27126938 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Thrombin
• protease-activated receptor 4
• Doxycycline

Topics

• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Colorectal Neoplasms (pathology)
• Down-Regulation
• Doxycycline (pharmacology)
• Humans
• Plasmids
• Receptors, Thrombin (genetics, physiology)