Abstract |
A method to determine residue concentrations of anti- coagulant rodenticides, brodifacoum (BF) and bromadiolone (BD) in liver was developed, using gas chromatography/mass spectrometry. Nine dogs were given 1.1 mg of BF/kg of body weight, PO, in polyethylene glycol 400, one time. Rats were fed BF or BD (via commercial baits) in amounts from 0.28 to 11.25 mg/kg over 1- to 4-day periods. Fresh liver samples were collected at necropsy from all rats and 3 dogs, ground with Na2SO4, and extracted with CHCl3:MeOH (9:1). After evaporation and silica cartridge purification were performed, residues were oxidized with a 0.16M chromic acid solution, and an oxidation product (4-bromobenzoic acid) was partitioned into CHCl3. The methylated derivative (port derivatization with trimethylanilinium hydroxide) was assayed, using gas chromatography/mass spectrometry. Bromadiolone was detected in livers from rats given greater than 6 mg of BD/kg of body weight, but not in livers of rats given 1.25 mg of BD/kg. In contrast, BF was detected (with one exception) in livers from dogs (given 1.1 mg of BF/kg) and from rats given high (11.25 mg of BF/kg) and low (0.28 mg of BF/kg) doses. This protocol, which does not differentiate between BF and BD because of the formation of a common product after chromic acid oxidation, was used to diagnose anticoagulant toxicosis in 3 dogs, 1 human being and 1 llama naturally poisoned.
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Authors | A C Ray, M J Murphy, M D DuVall, J C Reagor |
Journal | American journal of veterinary research
(Am J Vet Res)
Vol. 50
Issue 4
Pg. 546-50
(Apr 1989)
ISSN: 0002-9645 [Print] United States |
PMID | 2712421
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 4-Hydroxycoumarins
- Pesticide Residues
- Rodenticides
- bromfenacoum
- bromadiolone
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Topics |
- 4-Hydroxycoumarins
(analysis, poisoning)
- Animals
- Camelids, New World
- Chromatography, Gas
- Dog Diseases
(diagnosis)
- Dogs
- Female
- Humans
- Liver
(analysis)
- Male
- Mass Spectrometry
- Pesticide Residues
(analysis)
- Rats
- Rats, Inbred Strains
- Rodenticides
(analysis, poisoning)
- Time Factors
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