Abstract |
Cysteine conjugate beta-lyase (beta- lyase) was purified to electrophoretic homogeneity from the kidney cytosol of male Wistar rats. The highly purified enzyme exhibited a monomeric molecular weight of 50,000 Da and was active in the alpha-beta elimination of cysteine conjugates including S-(1,2-dichlorovinyl)-L-cysteine (DCVC), S-(1,1,2,2-tetrafluoroethyl)-L-cysteine (TFEC), and S-(2-benzothiazolyl)-L-cysteine, particularly toward DCVC and TFEC. The purified enzyme also exhibited glutamine transaminase K activity with phenylalanine and alpha-keto-gamma-methiolbutyrate as substrates. An antibody was raised to the purified rat protein in sheep and the crude immune serum affinity purified, yielding a specific antibody that recognized only the beta- lyase protein in whole kidney homogenates. Immunocytochemical studies on rat kidney sections stained with the purified antibody revealed that the cytosolic beta- lyase enzyme was mainly localized in the pars recta of the proximal tubule in untreated rats. This localization is coincident with the site-specific kidney necrosis produced by hexachloro-1,3-butadiene (HCBD). These results indicate that the tissue localization of beta- lyase in the proximal tubule plays an important role in determining the specific nephrotoxicity produced by halogenated alkenes such as HCBD.
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Authors | M MacFarlane, J R Foster, G G Gibson, L J King, E A Lock |
Journal | Toxicology and applied pharmacology
(Toxicol Appl Pharmacol)
Vol. 98
Issue 2
Pg. 185-97
(Apr 1989)
ISSN: 0041-008X [Print] United States |
PMID | 2711386
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Butadienes
- hexachlorobutadiene
- Transaminases
- glutamine - phenylpyruvate transaminase
- kynurenine-oxoglutarate transaminase
- Lyases
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Topics |
- Animals
- Antibody Specificity
- Butadienes
(metabolism, toxicity)
- Cross Reactions
- Cytosol
(enzymology)
- Immunohistochemistry
- Kidney
(drug effects, enzymology)
- Lyases
(analysis, immunology, isolation & purification)
- Male
- Molecular Weight
- Rats
- Rats, Inbred Strains
- Transaminases
(analysis, immunology, isolation & purification)
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