The utility of azan trichrome staining in Ameloblastoma.

Abstract

BACKGROUND:

It is occasionally difficult to distinguish the stellate reticulum-like region of ameloblastoma from the fibrous connective tissue stroma. This difficulty is further pronounced in the plexiform variant of ameloblastoma that has very sparse fibrous connective tissue.

AIM:

To test the utility of Azan trichrome stain in marking tumour regions and the peri-tumour environment of ameloblastoma.

MATERIALS AND METHODS:

Sections were prepared for 18 formalin fixed paraffin-embedded blocks of ameloblastoma cases and stained with Azan trichrome stain according to the manufacturer's specification.

RESULTS AND CONCLUSIONS:

The tumour areas were stained mostly brown, with the ameloblasts mainly marked as deep brown while the stellate reticulum-like region was light brown. The structures in the peri-tumour region were marked with different shades of blue. Azan trichrome staining was able to distinguish between the fibrous connective tissue and the stellate reticulum-like areas in 100% of the cases.

• Authors: Akinyele O Adisa, Samuel E Udeabor, Alica Kubesch, Mike Barbeck, Shahram Ghanaati
• Journal: The Nigerian postgraduate medical journal (Niger Postgrad Med J) 2016 Jan-Mar Vol. 23 Issue 1 Pg. 44-6 ISSN: 1117-1936 [Print] Nigeria
• PMID: 27098950 (Publication Type: Journal Article)

Topics

• Ameloblastoma (diagnosis)
• Animals
• Humans
• Nigeria
• Staining and Labeling