Derivatization and high-performance liquid chromatographic determination of urinary glycolic acid.

Abstract

A high-performance liquid chromatographic method for the determination of urinary glycolic acid is proposed, based on pre-column derivatization with phenylhydrazine coupled with the enzymatic oxidation of glycolate to glyoxylate. The phenylhydrazone formed is separated by liquid chromatography and detected at 324 nm. The minimum detectable concentration of glycolate was 10.0 mumol/l. The recovery of glycolate added to urine averaged 96.1%. The day-to-day coefficients of variation calculated by analysis of two urine samples with normal and high glycolate contents were 4.6 and 7.5%, respectively. Results of analyses of urine samples from healthy persons, idiopathic calcium stone formers and Type I primary hyperoxaluria patients are reported.

• Authors: M Petrarulo, S Pellegrino, O Bianco, M Marangella, F Linari, E Mentasti
• Journal: Journal of chromatography (J Chromatogr) Vol. 465 Issue 1 Pg. 87-93 (Mar 10 1989) Netherlands
• PMID: 2708493 (Publication Type: Journal Article)

Chemical References

• Glycolates
• Glyoxylates
• Hydrazones
• phenylhydrazone
• glycolic acid
• glyoxylic acid

Topics

• Chromatography, High Pressure Liquid
• Glycolates (urine)
• Glyoxylates (metabolism)
• Humans
• Hydrazones (urine)
• Hyperoxaluria (urine)