TNF-α and intPLA2 genes' polymorphism in anorexia nervosa.

Abstract

OBJECTIVE:

The aim of this study was the assessment of -308G/A tumor necrosis factor (TNF)-α gene polymorphism and intPLA2 gene polymorphism in patients with anorexia nervosa (AN) and healthy controls.

SUBJECTS:

We studied 91 non-related patients with AN and 144 healthy women (blood donors and students). The mean age of women from study group was 18.22 years (SD ± 3.13 years) and from control group was 31.71 years (SD ± 8.22).

METHODS:

Gene polymorphisms were studied with the use of polymerase chain reaction-restriction fragment length polymorphism method. TNF-α gene polymorphism consists of G/A substitution in -308 promoter region. IntPLA2 gene polymorphism is related to intron 1, in which restrictive region is found and recognized by BanI enzyme.

RESULTS:

We did not obtain statistically significant differences in the frequency of genotypes and alleles of -308G/A TNF-α polymorphism between the study and control groups (genotypes: P = 0.106, alleles: P = 0.076). We did analogous analysis in the restrictive and bulimic subgroups. We did not observe statistically relevant differences in the frequency of genotypes (P = 0.700) and alleles (P = 0.305). We did not obtain statistically relevant difference in the frequency of genotypes and alleles of intPLA2 gene between the study group and controls (genotypes: P = 0.300, alleles: P = 0.331). We did analogous analysis in both subgroups of AN. We did not observe statistically relevant differences in the frequency of genotypes (P = 0.344) and alleles (P = 0.230).

CONCLUSIONS:

There was no statistically relevant trend for the association between TNF-α polymorphism and AN. We did not find association between studied polymorphism of intPLA2 gene and risk of AN.

• Authors: Agnieszka Slopien, Filip Rybakowski, Monika Dmitrzak-Weglarz, Piotr Czerski, Joanna Hauser, Renata Komorowska-Pietrzykowska, Andrzej Rajewski
• Journal: Acta neuropsychiatrica (Acta Neuropsychiatr) Vol. 16 Issue 6 Pg. 290-4 (Dec 2004) ISSN: 0924-2708 [Print] England
• PMID: 26984541 (Publication Type: Journal Article)