[Immunoenzyme analysis with corpuscular antigen in the study of zoonotic cutaneous leishmaniasis].

Abstract

Comparative study of sera from patients suffering from zoonotic cutaneous leishmaniasis by indirect immunofluorescence (IIF) and by enzyme immunoassay (ELISA) gave 72.4 and 89.6% positive results, correspondingly, with homologous antigen L. major. Good correlation of these methods was revealed--coincidence in 83% cases. Authors used ELISA with corpuscular Leishmania antigen. The test was superior to IIF in sensitivity and specificity. Creation of type-specific Leishmania test systems for ELISA opens new vistas in studies of the population immune structure in combined zoonotic cutaneous leishmaniasis and visceral leishmaniasis foci.

• Authors: D B Goncharov, L I Gracheva, V M Saf'ianova, R S Dobrzhanskaia, Kh Kh Khuseĭinova
• Journal: Meditsinskaia parazitologiia i parazitarnye bolezni (Med Parazitol (Mosk)) 1989 Nov-Dec Issue 6 Pg. 31-5 ISSN: 0025-8326 [Print] Russia (Federation)
• Vernacular Title: Immunofermentnyĭ analiz s korpuskuliarnym antigenom pri izuchenii zoonoznogo kozhnogo leĭshmanioza.
• PMID: 2697793 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Antibodies, Protozoan
• Antigens, Protozoan

Topics

• Animals
• Antibodies, Protozoan (analysis)
• Antigens, Protozoan
• Enzyme-Linked Immunosorbent Assay (instrumentation, methods)
• Erythrocytes (immunology)
• Evaluation Studies as Topic
• Fluorescent Antibody Technique (instrumentation)
• Humans
• Leishmania tropica (immunology)
• Leishmaniasis (diagnosis)