[Effect of Conditioned Medium from Endothelial Cells on Cancer Stem Cell Phenotype of Hepatoma Cells].

Abstract

In this study, we aimed to investigate the influences of conditioned medium from human umbilical vein endothelial cells (HUVEC) on cancer stem cell phenotype of human hepatoma cells. HUVEC and human hepatoma cells (MHCC97H) were cultured, respectively, and then the MHCC97H cells were co-cultured with conditioned medium from HUVEC (EC-CM) with Transwell system. Anti-cancer drug sensitivity, colony-formation, migration/invasion ability, expression of cancer stem cell marker and sphere formation were performed to determine the cancer stem cell phenotype in MHCC97H cells. We found that MHCC97H cells co-cultured with EC-CM exhibited significantly higher colony-formation ability and lower sensitivity of anti-cancer drugs 5-FU and Cis. Transwell assay showed that treatment with EC-CM obviously increased migration and invasion of MHCC97H cells. Moreover, increased sphere forming capability and expression of CD133 in MHCC97H cells were observed after co-cultured with EC-CM. These results suggested that EC-CM could promote cancer stem cell phenotype of hepatoma cells.

• Authors: Chuan Feng, Xianjiong Yang, Jinghui Sun, Qing Luo, Guanbin Song
• Journal: Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi (Sheng Wu Yi Xue Gong Cheng Xue Za Zhi) Vol. 32 Issue 5 Pg. 1061-6 (Oct 2015) ISSN: 1001-5515 [Print] China
• PMID: 26964312 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Culture Media, Conditioned
• Fluorouracil

Topics

• Antineoplastic Agents (pharmacology)
• Carcinoma, Hepatocellular
• Cell Line, Tumor
• Coculture Techniques
• Culture Media, Conditioned
• Fluorouracil (pharmacology)
• Human Umbilical Vein Endothelial Cells (chemistry)
• Humans
• Liver Neoplasms
• Neoplastic Stem Cells (cytology)
• Phenotype