Development of an effective delivery system for intranasal immunization against Mycobacterium tuberculosis ESAT-6 antigen.

Abstract

Introduction The early secreted antigenic target 6-kDa protein (ESAT-6) plays an important role in immune protection against Tuberculosis. Owing to its great potential to increase immune response, chitosan can be considered as a suitable biodegradable polymer for intranasal administration. Methods The physiochemical properties of the nanoparticle were measured in vitro. Two weeks after the last intranasal administration, blood samples were collected and specific IgG, IFN-gama, and IL-4 levels were measured by ELISA. Results Chitosan nanoparticles containing ESAT-6 demonstrated stronger ability to induce IFN-gama, IL-4, and IgG antibody level than the control groups. Conclusion Administration of chitosan nanoparticles can be a suitable method to induce more appropriate immune responses against low inherent immunogenic tuberculosis proteins through intranasal routs.

• Authors: Yousef Amini, Majid Tebianian, Nader Mosavari, Mahdi Fasihi Ramandi, Seyyd Mahmoud Ebrahimi, Hamid Najminejad, Mehran Dabaghian, Meghdad Abdollahpour
• Journal: Artificial cells, nanomedicine, and biotechnology (Artif Cells Nanomed Biotechnol) Vol. 45 Issue 2 Pg. 291-296 (Mar 2017) ISSN: 2169-141X [Electronic] England
• PMID: 26924121 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Proteins
• ESAT-6 protein, Mycobacterium tuberculosis
• IFNG protein, mouse
• Immunoglobulin G
• Interleukin-4
• Interferon-gamma

Topics

• Administration, Intranasal (methods)
• Animals
• Antibodies, Bacterial (blood, immunology)
• Antigens, Bacterial (immunology, pharmacology)
• Bacterial Proteins (immunology, pharmacology)
• Drug Delivery Systems (methods)
• Female
• Immunization (methods)
• Immunoglobulin G (blood, immunology)
• Interferon-gamma (blood, immunology)
• Interleukin-4 (blood, immunology)
• Mice
• Mice, Inbred BALB C
• Mycobacterium tuberculosis (immunology)