Effects of bradykinin, GTP gamma S, R59022 and N-ethylmaleimide on inositol phosphate production in NG108-15 cells.

Abstract

Accumulation of inositol phosphates in NG108-15 neuroblastoma x glioma hybrid cells, pre-labeled for 24h to equilibrium, was stimulated by bradykinin, guanosine 5'-O-(3-thiotriphosphate) and the diacylglycerol kinase inhibitor R59022. Only the stimulation by bradykinin was inhibited by the bradykinin receptor antagonist [D-Arg0, Hyp3, Phe7, Thi5,8] bradykinin. Neither bradykinin nor R059022 increased the labeling of the inositol phospholipids. The sulfhydryl-alkylating reagent N-ethylmaleimide at 100 microM essentially abolished the stimulation caused by all three agents, possibly by preventing the binding of GTP to a guanine nucleotide-binding regulatory protein of as yet unknown size.

• Authors: C F Chiang, G Hauser
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 165 Issue 1 Pg. 175-82 (Nov 30 1989) ISSN: 0006-291X [Print] United States
• PMID: 2686644 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Inositol Phosphates
• Platelet Activating Factor
• Pyrimidinones
• Thiazoles
• Thionucleotides
• Guanosine 5'-O-(3-Thiotriphosphate)
• Inositol
• Guanosine Triphosphate
• R 59022
• Ethylmaleimide
• Bradykinin

Topics

• Animals
• Bradykinin (pharmacology)
• Cell Line
• Drug Interactions
• Ethylmaleimide (pharmacology)
• Glioma
• Guanosine 5'-O-(3-Thiotriphosphate)
• Guanosine Triphosphate (pharmacology)
• Hybrid Cells (drug effects, metabolism)
• Inositol (metabolism)
• Inositol Phosphates (metabolism)
• Kinetics
• Mice
• Neuroblastoma
• Platelet Activating Factor (antagonists & inhibitors)
• Pyrimidinones (pharmacology)
• Rats
• Thiazoles (pharmacology)
• Thionucleotides (pharmacology)