Effects of bradykinin, GTP gamma S, R59022 and N-ethylmaleimide on inositol phosphate production in NG108-15 cells.

Accumulation of inositol phosphates in NG108-15 neuroblastoma x glioma hybrid cells, pre-labeled for 24h to equilibrium, was stimulated by bradykinin, guanosine 5'-O-(3-thiotriphosphate) and the diacylglycerol kinase inhibitor R59022. Only the stimulation by bradykinin was inhibited by the bradykinin receptor antagonist [D-Arg0, Hyp3, Phe7, Thi5,8] bradykinin. Neither bradykinin nor R059022 increased the labeling of the inositol phospholipids. The sulfhydryl-alkylating reagent N-ethylmaleimide at 100 microM essentially abolished the stimulation caused by all three agents, possibly by preventing the binding of GTP to a guanine nucleotide-binding regulatory protein of as yet unknown size.
AuthorsC F Chiang, G Hauser
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 165 Issue 1 Pg. 175-82 (Nov 30 1989) ISSN: 0006-291X [Print] UNITED STATES
PMID2686644 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Inositol Phosphates
  • Platelet Activating Factor
  • Pyrimidinones
  • Thiazoles
  • Thionucleotides
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Inositol
  • Guanosine Triphosphate
  • R 59022
  • Ethylmaleimide
  • Bradykinin
  • Animals
  • Bradykinin (pharmacology)
  • Cell Line
  • Drug Interactions
  • Ethylmaleimide (pharmacology)
  • Glioma
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Guanosine Triphosphate (pharmacology)
  • Hybrid Cells (drug effects, metabolism)
  • Inositol (metabolism)
  • Inositol Phosphates (metabolism)
  • Kinetics
  • Mice
  • Neuroblastoma
  • Platelet Activating Factor (antagonists & inhibitors)
  • Pyrimidinones (pharmacology)
  • Rats
  • Thiazoles (pharmacology)
  • Thionucleotides (pharmacology)

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