Formation mechanism and biological activity of novel thiolated human-like collagen iron complex.

Abstract

To develop an iron supplement that is effectively absorbed and utilized, thiolated human-like collagen was created to improve the iron binding capacity of human-like collagen. A thiolated human-like collagen-iron complex was prepared in a phosphate buffer, and one mole of thiolated human-like collagen-iron possessed approximately 28.83 moles of iron. The characteristics of thiolated human-like collagen-iron were investigated by ultraviolet-visible absorption spectroscopy, Fourier transform infrared spectroscopy, circular dichroism, and differential scanning calorimetry. The results showed that the thiolated human-like collagen-iron complex retained the secondary structure of human-like collagen and had greater thermodynamic stability than human-like collagen, although interactions between iron ions and human-like collagen occurred during the formation of the complex. In addition, to evaluate the bioavailability of thiolated human-like collagen-iron, an in vitro Caco-2 cell model and an in vivo iron deficiency anemia mouse model were employed. The data demonstrated that the thiolated human-like collagen-iron complex exhibited greater bioavailability and was more easily utilized than FeSO4, ferric ammonium citrate, or ferrous glycinate. These results indicated that the thiolated human-like collagen-iron complex is a potential iron supplement in the biomedical field.

• Authors: Chenhui Zhu, Lingyun Liu, Jianjun Deng, Xiaoxuan Ma, Junfeng Hui, Daidi Fan
• Journal: Journal of biomaterials applications (J Biomater Appl) Vol. 30 Issue 8 Pg. 1205-18 (Mar 2016) ISSN: 1530-8022 [Electronic] England
• PMID: 26847914 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2016.

Chemical References

• Coordination Complexes
• Sulfhydryl Compounds
• Collagen
• Iron

Topics

• Anemia, Iron-Deficiency (drug therapy, metabolism)
• Animals
• Binding Sites
• Biological Availability
• Caco-2 Cells
• Collagen (chemistry, pharmacokinetics, therapeutic use)
• Coordination Complexes (chemistry, pharmacology, therapeutic use)
• Humans
• Iron (chemistry, pharmacokinetics, therapeutic use)
• Male
• Mice
• Sulfhydryl Compounds (chemistry, pharmacokinetics, therapeutic use)