HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Biological consequences of a reduction in the non-target DNA scanning capacity of a DNA repair enzyme.

Abstract
Numerous DNA-interactive proteins have been shown to locate specific sequences within large domains of non-target DNA in vitro and in vivo by a one-dimensional diffusion mechanism; however, the biological significance of this process has not been evaluated. We have examined the biological consequences of sliding for the pyrimidine dimer-specific DNA repair enzyme T4 endonuclease V, an enzyme which scans non-target DNA both in vitro and in vivo. An endonuclease V mutant was constructed whose only altered biochemical characteristic, measured in vitro, was a loss in its ability to slide on non-target DNA. In contrast to the native enzyme, when the mutated endonuclease V was expressed in DNA repair-deficient Escherichia coli, no enhanced ultraviolet survival was conferred. These results suggest that the mechanisms which DNA-interactive proteins employ to enhance the probability of locating their target sequences are of significant biological importance.
AuthorsD R Dowd, R S Lloyd
JournalJournal of molecular biology (J Mol Biol) Vol. 208 Issue 4 Pg. 701-7 (Aug 20 1989) ISSN: 0022-2836 [Print] Netherlands
PMID2681789 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Bacterial
  • Pyrimidines
  • Endodeoxyribonucleases
  • deoxyribonuclease V
  • pyrimidine
Topics
  • DNA Repair
  • DNA, Bacterial (metabolism)
  • Endodeoxyribonucleases (metabolism)
  • Escherichia coli
  • Mutation
  • Pyrimidines

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: