IgG immunoadsorption in experimental allergic neuritis: effect on antibody levels and clinical course.

Abstract

The effect of IgG immunoadsorption upon the course of chronic experimental allergic neuritis (EAN) is described. Miniature membrane plasma separators coupled with a Protein A (PA)-Sepharose immunoadsorbent column were used to perform upon conscious rabbits 5 IgG immunoadsorption treatments over 6 days. Quantitation of anti-myelin IgG and IgM by ELISA revealed that 55-65% of plasma IgG was removed per treatment. Rapid post-treatment antibody rebound was observed for anti-myelin IgG although no antibody overshoot above control levels could be observed. Anti-myelin IgM levels remained relatively unaffected by PA immunoadsorption. Comparisons of clinical scores between control and treatment animals showed that IgG immunoadsorption was significantly beneficial (day 1 post-treatment p less than 0.001; day 2 post-treatment p less than 0.05). However, rapid relapse was observed in all treatment animals such that by day 3 post-treatment no significant clinical difference between control and treatment groups could be observed. IgG immunoadsorption suppresses the clinical progression of chronic EAN in a manner similar to that seen with plasma exchange. This finding suggests that antibody modulates early disease pathogenesis.

• Authors: G K Harvey, K Schindhelm, J D Pollard
• Journal: Journal of neurology, neurosurgery, and psychiatry (J Neurol Neurosurg Psychiatry) Vol. 52 Issue 7 Pg. 865-70 (Jul 1989) ISSN: 0022-3050 [Print] England
• PMID: 2671264 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantibodies
• Immunoglobulin G
• Immunoglobulin M

Topics

• Animals
• Autoantibodies (analysis)
• Female
• Immunoglobulin G (analysis)
• Immunoglobulin M (analysis)
• Immunosorbent Techniques
• Myelin Sheath (immunology)
• Neuritis, Autoimmune, Experimental (immunology, therapy)
• Rabbits