Abstract | BACKGROUND: METHODS: Thirty SLE patients were included in this study. CD4+ T cells were isolated from healthy subjects, and dendritic cells (DCs) were isolated from healthy subjects or SLE patients. C4 was supplemented to co-incubate with T cells and DCs. RESULTS: Serum C4 concentration was positively correlated with regulatory T cell (Treg) percentage (R(2) = 0.5907, p < 0.001) and TGFβ concentration (R(2) = 0.5641, p < 0.001) in SLE patients. Different concentrations of C4 had no effect on T cells differentiation. Co-incubated T cells with DCs and C4 for 7 days, the Treg percentage and TGF-β concentration were significantly elevated. In addition, pre-treated DCs (from healthy subjects or SLE patients) with C4 and then co-incubated with T cells, the increases of Treg percentage and TGF-β concentration were also observed. CONCLUSION: C4 takes part in T cells differentiation to Treg cells via DCs.
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Authors | Hong-Bin Cheng, Rong-Yi Chen, Jing-Ping Wu, Li Chen, Yan-Hua Liang, Hai-Feng Pan, Zi-Feng Pan, Qing-Hua Zhang, Qing Li, Tian-Xi Du, Yong-Mei Lv, Jian-Qiang Shi |
Journal | Cell & bioscience
(Cell Biosci)
Vol. 5
Pg. 73
( 2015)
ISSN: 2045-3701 [Print] England |
PMID | 26705467
(Publication Type: Journal Article)
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