Abstract | OBJECTIVE: PATIENTS AND METHODS: Cervical tumor specimens from 30 patients were recruited in this study. We analyzed the expression of cyclin I by real-time polymerase chain reaction (qRT-PCR), Western blotting examination of downstream effectors. Cell proliferation assay and xenograft experiments were performed for cisplatin cytotoxicity assay. Lentivirus-mediated and siRNA-mediated genes overexpression or knockdown were applied to investigate the role of cyclin I to cisplatin resistance in CC cell. RESULTS: CONCLUSIONS: These data suggest that a cyclin I-Cdk5 complex forms a critical antiapoptotic factor in the process of generating cisplatin resistance in cervical cancer.
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Authors | R Li, G-Z Liu, S-Y Luo, R Chen, J-X Zhang |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 19
Issue 23
Pg. 4533-41
(Dec 2015)
ISSN: 2284-0729 [Electronic] Italy |
PMID | 26698249
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Cyclin I
- RNA, Small Interfering
- Cyclin-Dependent Kinase 5
- CDK5 protein, human
- Cisplatin
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Topics |
- Animals
- Antineoplastic Agents
(therapeutic use)
- Apoptosis
(drug effects, genetics)
- Carcinoma, Squamous Cell
(drug therapy, genetics)
- Cisplatin
(therapeutic use)
- Cyclin I
(antagonists & inhibitors, genetics)
- Cyclin-Dependent Kinase 5
(genetics, metabolism)
- Drug Resistance, Neoplasm
(drug effects, genetics)
- Female
- Gene Expression Regulation, Neoplastic
(drug effects)
- HeLa Cells
- Humans
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- RNA, Small Interfering
(pharmacology)
- Uterine Cervical Neoplasms
(drug therapy, genetics)
- Xenograft Model Antitumor Assays
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