Anti-Obesity Effect of 6,8-Diprenylgenistein, an Isoflavonoid of Cudrania tricuspidata Fruits in High-Fat Diet-Induced Obese Mice.

Abstract

Obesity, which is characterized by excessive fat accumulation, is associated with several pathological disorders, including metabolic diseases. In this study, the anti-obesity effect of 6,8-diprenylgenistein (DPG), a major isoflavonoid of Cudrania tricuspidata fruits was investigated using high fat-diet (HFD)-induced obese mice at the doses of 10 and 30 mg/kg for six week. The body weight of the DPG-treated groups was significantly lower compared to the HFD-treated group. In addition, fat accumulation in epididymal adipose tissue and liver was dramatically decreased in the HFD + DPG groups. The food efficiency ratios of the HFD + DPG groups were also lower compared to the HFD group with the same food intake. Metabolic parameters that had increased in the HFD group were decreased in the HFD + DPG groups. Further studies demonstrate that DPG efficiently reduces lipogenic genes by regulation of transcription factors, such as peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), and hormones, such as leptin and adiponection. DPG also regulates acetyl-CoA carboxylase (ACC) and hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) by AMP-activated protein kinase (AMPK) activation. Taken together, DPG is beneficial for the regulation of obesity, especially resulting from high fat intake.

• Authors: Yang Hee Jo, Kyeong-Mi Choi, Qing Liu, Seon Beom Kim, Hyeong-Jin Ji, Myounghwan Kim, Sang-Kyung Shin, Seon-Gil Do, Eunju Shin, Gayoung Jung, Hwan-Soo Yoo, Bang Yeon Hwang, Mi Kyeong Lee
• Journal: Nutrients (Nutrients) Vol. 7 Issue 12 Pg. 10480-90 (Dec 15 2015) ISSN: 2072-6643 [Electronic] Switzerland
• PMID: 26694457 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 6,8-diprenylgenistein
• Adiponectin
• Anti-Obesity Agents
• CCAAT-Enhancer-Binding Protein-alpha
• Isoflavones
• Leptin
• PPAR gamma
• Plant Extracts
• Genistein
• Hydroxymethylglutaryl CoA Reductases
• AMP-Activated Protein Kinases
• Acetyl-CoA Carboxylase

Topics

• AMP-Activated Protein Kinases (genetics, metabolism)
• Acetyl-CoA Carboxylase (genetics, metabolism)
• Adiponectin (metabolism)
• Adipose Tissue (drug effects, metabolism)
• Animals
• Anti-Obesity Agents (pharmacology)
• CCAAT-Enhancer-Binding Protein-alpha (genetics, metabolism)
• Diet, High-Fat
• Fruit (chemistry)
• Gene Expression Regulation
• Genistein (analogs & derivatives, pharmacology)
• Hydroxymethylglutaryl CoA Reductases (genetics, metabolism)
• Isoflavones (pharmacology)
• Leptin (metabolism)
• Liver (drug effects, metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Obese
• Moraceae (chemistry)
• Obesity (drug therapy)
• PPAR gamma (genetics, metabolism)
• Plant Extracts (pharmacology)