Design of a Hyperpolarized Molecular Probe for Detection of Aminopeptidase N Activity.

Abstract

Aminopeptidase N (APN) is an important enzyme that is involved in tumor angiogenesis. Detection of APN activity can thus lead to early diagnosis and elucidation of tumor development. Although some molecular probes for APN have been developed, the detection of APN activity in opaque biological samples remains a challenge. To this end, we designed a hyperpolarized NMR probe [1-(13) C]Ala-NH2 which satisfies the prerequisites for APN detection, namely, sufficient retention of the hyperpolarized state, a high reactivity to APN, and an APN-induced chemical shift change. The [1-(13) C]Ala-NH2 probe allowed sensitive detection of APN activity using (13) C NMR spectroscopy.

• Authors: Ryunosuke Hata, Hiroshi Nonaka, Yoichi Takakusagi, Kazuhiro Ichikawa, Shinsuke Sando
• Journal: Angewandte Chemie (International ed. in English) (Angew Chem Int Ed Engl) Vol. 55 Issue 5 Pg. 1765-8 (Jan 26 2016) ISSN: 1521-3773 [Electronic] Germany
• PMID: 26689297 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Molecular Probes
• CD13 Antigens

Topics

• CD13 Antigens (metabolism)
• Kinetics
• Molecular Probes (chemistry)