[Mechanism of sophocarpine in treating experimental colitis in mice].

Abstract

To study the preventive effect of sophocarpine (Soc) on dextran sulfate sodium (DSS)-induced colitis in mice, in order to analyze the influence of Soc on toll like receptor 4 (TLR4)/mitogen-activated protein kinases (MAPKs) and janus tyrosine kinase 2 signal transducer and activator of transcription 3 (JAK2/STAT3) signal pathways in mice intestinal tissues. The mice was given 2.5% DSS for 6 days to induce the acute colitis model. The Soc-treated group was intraperitoneally injected with sophocarpine 30 mg · kg(-1) · d(-1) since the day before the experiment to the end. The disease activity index (DAI) was assessed everyday, and the colonic morphology and histological damage were observed with HE staining. The mRNA expressions of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were detected by real-time RT-PCR. The changes in key protein kinase p38 mitogen-activated protein kinase (p38MAPK), c-Jun NH2-terminal protein kinase1/2 (JNK1/2), extracellular signal-regulated kinase1/2 (ERK1/2), JAK2, STAT3 in TLR4/MAPKs and JAK2/STAT3 signaling pathways were detected by western blot. The result showed that the model group showed statistical significance in body weight, DAI, colon length and histopathological changes compared with the normal group (P <0.05); however, the Soc-treated group showed significant improvements in the above indexes compared with the model group (P <0.05). TNF-α, IL-1β and IL-6 in the model group was significantly higher than that in the normal group (P <0.05), but lowered in the Soc-treated group to varying degrees (P <0.05). In the normal group, the expressions of TLR4 and the phosphorylation of P38, JNK1/2, JAK2, STAT3 were at low levels; in the model group, the phosphorylation of P38, JNK1/2, JAK2, STAT3 increased; the Soc-treated group showed a decrease in TLR4 expression compared with the model group, with notable declines in the phosphorylation of TLR4, P38, JNK1/2, JAK2, STAT3. These findings indicate that Soc can inhibit TLR4/MAPKs, K2/STAT3 signaling pathway activation, reduce the expression of proinflammatory cytokines TNF-α, IL-1β and IL-6 and relieve inflammatory reactions, so as to effectively prevent experimental colitis.

• Authors: Jian-mei Zhang, Ya-bi Zhu, Xing Deng, Chang-xiong Wang, Shuang-mei Luan, Yue-xiang Chen
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 40 Issue 15 Pg. 3081-7 (Aug 2015) ISSN: 1001-5302 [Print] China
• PMID: 26677715 (Publication Type: English Abstract, Journal Article)

Chemical References

• Alkaloids
• Cytokines
• STAT3 Transcription Factor
• Stat3 protein, mouse
• Tlr4 protein, mouse
• Toll-Like Receptor 4
• sophocarpine
• Jak2 protein, mouse
• Janus Kinase 2

Topics

• Alkaloids (pharmacology, therapeutic use)
• Animals
• Colitis (drug therapy, immunology, pathology)
• Cytokines (genetics)
• Janus Kinase 2 (antagonists & inhibitors, physiology)
• Male
• Mice
• Mice, Inbred BALB C
• Phosphorylation
• STAT3 Transcription Factor (antagonists & inhibitors, physiology)
• Toll-Like Receptor 4 (antagonists & inhibitors, physiology)