[Effect of puerarin on hypoxia induced proliferation of PASMCs by regulating reactive oxygen].

Abstract

To discuss the effect of puerarin (Pue) on the proliferation of hypoxia-induced pulmonary artery smooth muscle cells (PASMCs) and discuss whether its mechanism is achieved by regulating reactive oxygen. PASMCs of primarily cultured rats (2-5 generations) were selected in the experiment. MTT, Western blot, FCM and DCFH-DA were used to observe Pue's effect the proliferation of PASMCs. The Western blot was adopted to detect whether ROS participated in Pue's effect in inhibiting PASMC proliferation. The PASMCs were divided into five groups: the normoxia group, the hypoxia group, the hypoxia + Pue group, the hypoxia + Pue + Rotenone group and the hypoxia + Rotenone group, with Rotenone as the ROS blocker. According to the results, under the conditions of normoxia, Pue had no effect on the PASMC proliferation; But, under the conditions of hypoxia, it could inhibit the PASMC proliferation; Under the conditions of normoxia and hypoxia, Pue had no effect on the expression of the tumor necrosis factor-α (TNF-α) among PASMCs, could down-regulate the expression of hypoxia-induced cell cycle protein Cyclin A and proliferative nuclear antigen (PCNA). DCFH-DA proved Pue could reverse ROS rise caused by hypoxia. Both Rotenone and Pue could inhibit the up-regulated expressions of HIF-1α, Cyclin A, PCNA caused by anoxia, with a synergistic effect. The results suggested that Pue could inhibit the hypoxia-induced PASMC proliferation. Its mechanism may be achieved by regulating ROS.

• Authors: Xiao-dan Zhang, Li-wei Wang, Shu-jing Wang, Da-ling Zhu, Yan-nan Yang, Jie-jing Sheng, Sha-sha Song
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 40 Issue 15 Pg. 3027-33 (Aug 2015) ISSN: 1001-5302 [Print] China
• PMID: 26677706 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Isoflavones
• Proliferating Cell Nuclear Antigen
• Reactive Oxygen Species
• puerarin

Topics

• Animals
• Cell Cycle (drug effects)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Hypoxia (pathology)
• Isoflavones (pharmacology)
• Male
• Myocytes, Smooth Muscle (drug effects, physiology)
• Proliferating Cell Nuclear Antigen (analysis)
• Pulmonary Artery (cytology, drug effects)
• Rats
• Rats, Wistar
• Reactive Oxygen Species (metabolism)