Abstract |
Increased expression of matrix metalloproteinase 13 (MMP13) in chondrocytes contributes to the development of osteoarthritis. The hypothesis of this study was that diet with a low ratio of n-6/n-3 polyunsaturated fatty acids (PUFAs) is associated with reduced MMP13 expression in inflammatory chondrocytes in vitro and in vivo. Human chondrocytes were cultured with different ratios of linoleic acid (LA, n-6 PUFA) to α- linolenic acid (n-3 PUFA) from 1:1 to 10:1. Proliferation of chondrocytes, MMP13 protein and mRNA levels were detected, respectively. Sprague-Dawley rats ( n=30) were fed diets containing different ratios of n-6/ n-3 PUFA. Freund's complete adjuvant was injected to make the model of arthritis. Paw swelling rate was measured and all rats were euthanized after 6 weeks of treatment. Serum MMP13 and IL-1 were measured by enzyme-linked immunosorbent assay. Joint histological sections were stained with safranin-O Fast Green to evaluate cartilage damage. Low ratio of LA/α- linolenic acid decreased the mRNA and protein levels of MMP13 but did not affect chondrocytes proliferation. Ratios of PUFA such as 1:1 and 2:1 significantly reduced paw swelling rate, and serum MMP13 and IL-1 levels in a rat model. Histological staining showed that ratios of 1:1 and 2:1 PUFA significantly alleviated cartilage damage in adjuvant-induced arthritis. A ratio of n-6/ n-3 PUFA of 1:1 showed the strongest inhibitory effect on MMP13. Our results indicate that a low ratio of n-6/ n-3 PUFA at 1:1 significantly suppressed MMP13 expression both in vitro and in vivo and reduced adjuvant-induced arthritis in rats could be a means to control and reduce the symptoms of osteoarthritis.
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Authors | Haomiao Yu, Yadong Li, Lifeng Ma, Hai Meng, Xiaodong Bai, Zihan Fan, Fei Yu, Ai Guo |
Journal | Nutrition research (New York, N.Y.)
(Nutr Res)
Vol. 35
Issue 12
Pg. 1113-21
(Dec 2015)
ISSN: 1879-0739 [Electronic] United States |
PMID | 26675329
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- Interleukin-1
- RNA, Messenger
- alpha-Linolenic Acid
- Linoleic Acid
- Matrix Metalloproteinase 13
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Topics |
- Animals
- Arthritis, Experimental
(blood, diet therapy, genetics, pathology)
- Cartilage
(drug effects, pathology)
- Cartilage Diseases
(blood, diet therapy, genetics, pathology)
- Chondrocytes
(drug effects)
- Edema
- Humans
- Interleukin-1
(blood)
- Joints
(drug effects, pathology)
- Linoleic Acid
(administration & dosage, pharmacology)
- Male
- Matrix Metalloproteinase 13
(blood, genetics)
- Osteoarthritis
(blood, diet therapy, genetics, pathology)
- RNA, Messenger
(metabolism)
- Rats, Sprague-Dawley
- alpha-Linolenic Acid
(administration & dosage, pharmacology)
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