Abstract | BACKGROUND: METHODS: We screened gelatinase inhibitor among Chinese herbal medicine by molecular docking technology; investigated the proliferation, migration and invasion of MDA-MB-231 human breast cancer cell line and 4T1 mouse breast cancer cell line in response to the treatment with the screened inhibitor by wound assay, invasion assay and gelatin zymography; then further examined the effects of inhibitor on allograft mammary tumors of mice by immunohistochemistry. RESULTS: We successfully screened an Chinese herbal medicine- Plantamajoside(PMS)-which can reduce the gelatinase activity of MMP9 and MMP2. In vitro, PMS can inhibit the proliferation, migration and invasion of MDA-MB-231 human breast cancer cell line and 4T1 mouse breast cancer cell line by decreasing MMP9 and MMP2 activity. In vivo, oral administration of PMS to the mice bearing 4T1 cells induced tumors resulted in significant reduction in allograft tumor volume and weights, significant decrease in microvascular density and significant lower lung metastasis rate. CONCLUSIONS: Our results indicate that as a promising anti- cancer agent, PMS may inhibit growth and metastasis of breast cancer by inhibiting the activity of MMP9 and MMP2.
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Authors | Shimin Pei, Xu Yang, Huanan Wang, Hong Zhang, Bin Zhou, Di Zhang, Degui Lin |
Journal | BMC cancer
(BMC Cancer)
Vol. 15
Pg. 965
(Dec 16 2015)
ISSN: 1471-2407 [Electronic] England |
PMID | 26674531
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Catechols
- Drugs, Chinese Herbal
- Glucosides
- Matrix Metalloproteinase Inhibitors
- plantamajoside
- Matrix Metalloproteinase 2
- Matrix Metalloproteinase 9
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Blotting, Western
- Breast Neoplasms
(enzymology, pathology)
- Catechols
(pharmacology)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Cell Proliferation
(drug effects)
- Drugs, Chinese Herbal
(pharmacology)
- Female
- Glucosides
(pharmacology)
- Humans
- Immunohistochemistry
- Lung Neoplasms
(secondary)
- Matrix Metalloproteinase 2
(metabolism)
- Matrix Metalloproteinase 9
(metabolism)
- Matrix Metalloproteinase Inhibitors
(pharmacology)
- Mice
- Mice, Inbred BALB C
- Neoplasm Invasiveness
(pathology)
- Phytotherapy
(methods)
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