No association of IL-12p40 pro1.1 polymorphism with juvenile idiopathic arthritis.

Abstract

BACKGROUND:

IL-12p40 plays an important role in the activation of the T-cell lines like Th17 and Th1-cells. Theses cells are crucial in the pathogenesis of juvenile idiopathic arthritis. A polymorphism in its promoter region and the genotype IL12p40 pro1.1 leads to a higher production of IL-12p40. We studied whether there is a difference in the distribution of the genotype in patients with JIA and the healthy population.

METHODS:

In 883 patients and 321 healthy controls the IL-12p40 promoter genotype was identified by ARMS-PCR.

RESULTS:

There is no association of IL-12p40 pro polymorphism neither in patients with JIA compared to controls nor in subtypes of JIA compared to oligoarthritis. We found a non-significant tendency of a higher prevalence of the genotype pro1.1 in systemic arthritis (32.4%) and in rheumatoid factor negative polyarthritis (30.5%) and a lower pro1.1 genotype in persistent oligoarthritis (20.7%) and in enthesitis-related arthritis (17%). Likelihood of the occurrence of genotype IL12-p40 pro1.1 in patients with systemic arthritis (OR 1.722, CI 95% 1.344-2.615, p 0.0129) and RF-negative polyarthritis (OR 1.576, CI 95% 1.046-2.376, p 0.0367) compared to persistent oligoarthritis was significantly higher. This was also true for comparison of their homozygous genotypes IL-12p40 pro 1.1 and 2.2 in systemic arthritis (OR 1.779, CI 95 % 1.045-3.029, p 0.0338). However, in Bonferroni correction for multiple hypothesis this was not significant.

CONCLUSION:

A tendency of a higher prevalence of the genotype IL-12p40 pro1.1 in systemic arthritis and in rheumatoid factor negative polyarthritis was observed but not significant. Further investigations should be done to clarify the role IL-12p40 in the different subtypes of JIA.

• Authors: Christiane S Eberhardt, Johannes-Peter Haas, Hermann Girschick, Tobias Schwarz, Henner Morbach, Angela Rösen-Wolff, Dirk Foell, Guenther Dannecker, Carsten Schepp, Gerd Ganser, Nora Honke, Thomas Eggermann, Jan Müller-Berghaus, Norbert Wagner, Kim Ohl, Klaus Tenbrock
• Journal: Pediatric rheumatology online journal (Pediatr Rheumatol Online J) Vol. 13 Pg. 61 (Dec 15 2015) ISSN: 1546-0096 [Electronic] England
• PMID: 26667304 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-12 Subunit p40

Topics

• Arthritis, Juvenile (genetics)
• Case-Control Studies
• Humans
• Interleukin-12 Subunit p40 (genetics)
• Polymerase Chain Reaction
• Polymorphism, Genetic (genetics)
• Promoter Regions, Genetic (genetics)