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The Role of Glutamate Dehydrogenase (GDH) Testing Assay in the Diagnosis of Clostridium difficile Infections: A High Sensitive Screening Test and an Essential Step in the Proposed Laboratory Diagnosis Workflow for Developing Countries like China.

Abstract
The incidence and severity of Clostridium difficile infection (CDI) in North America and Europe has increased significantly since the 2000s. However, CDI is not widely recognized in China and other developing countries due to limited laboratory diagnostic capacity and low awareness. Most published studies on laboratory workflows for CDI diagnosis are from developed countries, and thus may not be suitable for most developing countries. Therefore, an alternative strategy for developing countries is needed. In this study, we evaluated the performance of the Glutamate Dehydrogenase (GDH) test and its associated workflow on 416 fecal specimens from suspected CDI cases. The assay exhibited excellent sensitivity (100.0%) and specificity (92.8%), compared to culture based method, and thus could be a good screening marker for C. difficile but not for indication of toxin production. The VIDAS CDAB assay, which can detect toxin A/B directly from fecal specimens, showed good specificity (99.7%) and positive predictive value (97.2%), but low sensitivity (45.0%) and negative predictive value (88.3%), compared with PCR-based toxin gene detection. Therefore, we propose a practical and efficient GDH test based workflow strategy for the laboratory diagnosis of CDI in developing countries like China. By applying this new workflow, the CDI laboratory diagnosis rate was notably improved in our center, yet the increasing cost was kept at a minimum level. Furthermore, to gain some insights into the genetic population structure of C. difficile isolates from our hospital, we performed MLST and PCR toxin gene typing.
AuthorsJing-Wei Cheng, Meng Xiao, Timothy Kudinha, Zhi-Peng Xu, Lin-Ying Sun, Xin Hou, Li Zhang, Xin Fan, Fanrong Kong, Ying-Chun Xu
JournalPloS one (PLoS One) Vol. 10 Issue 12 Pg. e0144604 ( 2015) ISSN: 1932-6203 [Electronic] United States
PMID26659011 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Bacterial Proteins
  • Bacterial Toxins
  • Glutamate Dehydrogenase
Topics
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Bacterial Proteins (analysis, biosynthesis, genetics)
  • Bacterial Toxins (analysis, biosynthesis)
  • Bacterial Typing Techniques (methods, standards)
  • Biological Assay
  • Child
  • Child, Preschool
  • China
  • Clostridioides difficile (genetics, isolation & purification, metabolism)
  • Developing Countries
  • Enterocolitis, Pseudomembranous (diagnosis, microbiology)
  • Feces (microbiology)
  • Female
  • Glutamate Dehydrogenase (analysis, biosynthesis, genetics)
  • Humans
  • Male
  • Middle Aged
  • Multilocus Sequence Typing
  • Polymerase Chain Reaction
  • Sensitivity and Specificity

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