[The expression and significance of hnRNPD in esophageal squamous cell carcinoma cells].

Abstract

OBJECTIVE:

To investigate the expression of heterogeneous nuclear ribonucleoprotein D (hnRNPD) in esophageal squamous cell carcinoma (ESCC) tissues and the relationship between hnRNPD expression and the clinicopathological features of ESCC, and to study the effect of down-regulated hnRNPD on the proliferation of ESCC cells and explore its potential mechanism.

METHODS:

The expression of hnRNPD protein in ESCC tissues and the normal paracancerous tissues were detected by immunohistochemistry. The siRNA-hnRNPD was transfected into ESCC cells and the silence effect was verified by Western blotting. MTT assay and clone formation assay were used to evaluate the proliferation of ESCC cells after down-regulation of hnRNPD genes. Cell apoptosis was examined by annexin V-phycoerythrin/7-aminoactinomycin D (annexin V-PE/7-AAD) staining and flow cytometry.

RESULTS:

The expression of hnRNPD protein in ESCC tissues was significantly higher than that of the normal paracancerous tissues, and the expression was closely related with neoplasm staging. Down-regulation of hnRNPD inhibited the proliferation and clonality of ESCC cells. Compared with the control group, siRNA targeting hnRNPD significantly promoted cell apoptosis.

CONCLUSION:

Down-regulation of hnRNPD inhibits the proliferation of ESCC cells by promoting cell apoptosis.

• Authors: Yangyang Geng, Lulu Zhang, Miaomiao Xu, Wenjiong Sheng, Aijing Dong, Jinming Cao, Jianping Cao
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 31 Issue 12 Pg. 1659-63 (Dec 2015) ISSN: 1007-8738 [Print] China
• PMID: 26648300 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Heterogeneous-Nuclear Ribonucleoprotein D

Topics

• Adult
• Aged
• Apoptosis
• Carcinoma, Squamous Cell (enzymology, genetics, physiopathology)
• Cell Line, Tumor
• Cell Proliferation
• Down-Regulation
• Esophageal Neoplasms (enzymology, genetics, physiopathology)
• Esophageal Squamous Cell Carcinoma
• Female
• Gene Expression Regulation, Neoplastic
• Heterogeneous-Nuclear Ribonucleoprotein D (genetics, metabolism)
• Humans
• Male
• Middle Aged