Sepsis is a complex condition with unacceptable mortality.
Betulin is a natural extract with multiple bioactivities. This study aims to evaluate the potential effects of
betulin on lung and liver injury in
sepsis. Cecal
ligation and
puncture was used to establish the rat model of
sepsis. A single dose of 4mg/kg or 8mg/kg
betulin was injected intraperitoneally immediately after the model establishment. The survival rate was recorded every 12h for 96h. The organ injury was examined using
hematoxylin and
eosin staining and serum biochemical test. The levels of proinflammatory
cytokines and high mobility group box 1 in the serum were measured using ELISA. Western blotting was used to detect the expression of
proteins in NF-κB and MAPK signaling pathways.
Betulin treatment significantly improved the survival rate of septic rats, and attenuated lung and liver injury in
sepsis, including the reduction of
lung wet/dry weight ratio and activities of
alanine aminotransferase and
aspartate aminotransferase in the serum. In addition, levels of
tumor necrosis factor-α, interleukin-1β,
interleukin-6 and high mobility group box 1 in the serum were also lowered by
betulin treatment. Moreover,
sepsis-induced activation of the NF-κB and MAPK signaling pathway was inhibited by
betulin as well. Our findings demonstrate the protective effect of
betulin in lung and liver injury in
sepsis. This protection may be mediated by its anti-inflammatory and NF-κB and MAPK inhibitory effects.