Abstract | BACKGROUND: METHODOLOGY/PRINCIPAL FINDINGS: The sequence of SjVAMP2 was analyzed, cloned, expressed and characterized. SjVAMP2 is a member of the synaptobrevin superfamily harboring the v-SNARE coiled-coil homology domain. RT-PCR analysis revealed that significantly higher SjVAMP2 levels were observed in 14-, 28- and 42-day-old worms, and SjVAMP2 expression was much higher in 42-day-old female worms than in those male worms. Additionally, the expression of SjVAMP2 was associated with membrane recovery in PZQ-treated worms. Immunostaining assay showed that SjVAMP2 was mainly distributed in the sub-tegument of the worms. Western blotting revealed that rSjVAMP2 showed strong immunogenicity. Purified rSjVAMP2 emulsified with ISA206 adjuvant induced 41.5% and 27.3% reductions in worm burden, and 36.8% and 23.3% reductions in hepatic eggs in two independent trials. Besides, significantly higher rSjVAMP2-specific IgG, IgG1, IgG2a levels were detected in rSjVAMP2-vaccinated mice. CONCLUSION: Our study indicated that SjVAMP2 is a potential vaccine candidate against S. japonicum and provided the basis for further investigations into the biological function of SjVAMP2.
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Authors | Qian Han, Yang Hong, Zhiqiang Fu, Min Zhang, Xiaodan Cao, Yantao Liu, Shuai Ma, Yuntao Guo, Ke Lu, Chuangang Zhu, Jiaojiao Lin |
Journal | PloS one
(PLoS One)
Vol. 10
Issue 12
Pg. e0144584
( 2015)
ISSN: 1932-6203 [Electronic] United States |
PMID | 26641090
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Helminth
- Helminth Proteins
- Recombinant Proteins
- Vesicle-Associated Membrane Protein 2
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Topics |
- Amino Acid Sequence
- Animals
- Antibodies, Helminth
(blood)
- Blotting, Western
- Female
- Helminth Proteins
(genetics, immunology)
- Male
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Phylogeny
- Real-Time Polymerase Chain Reaction
- Recombinant Proteins
(genetics, immunology)
- Schistosoma japonicum
(genetics, immunology)
- Schistosomiasis japonica
(parasitology, prevention & control)
- Sequence Homology, Amino Acid
- Vaccination
- Vesicle-Associated Membrane Protein 2
(genetics, immunology)
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